1) A phlebotomist fails to check patient identifiers before collecting blood samples. What is the most likely consequence?
Clotting
Hemolysis
Increased turnaround time
Specimen misidentification
2) A patient arrives for a blood draw when the phlebotomist realizes she forgot to label the collection tubes from the previous patient, who has already left. What should the phlebotomist do?
Discard the tubes and schedule a redraw
Label the tubes immediately using the information from requisition form
Send the unlabelled tubes to the laboratory with a note of explanation
Set the tubes aside and label them later
3) A phlebotomist accidentally collects a coagulation test in a lavender-top tube instead of a light blue-top tube. What should the phlebotomist do?
Add sodium citrate to the red-top tube
Pour the blood sample into the correct tube
Reject the specimen and recollect it in the correct tube
Relabel the lavender-top tube as a light blue-top tube
4) A technologist notes a clot in an EDTA sample. What is the most appropriate action?
Dilute the sample to account for the trapped cells
Gently stir the sample to break up the clot
Reject the specimen and request a new one
Remove the clot with an applicator stick
5) You are labeling a specimen when a coworker interrupts you to ask a question. What is the best action?
Answer your coworker's question first
Ask someone else to label the specimen
Label the specimen first
Redraw the specimen
6) How much water must be added to 1 liter of 85% v/v alcohol to dilute it to 70%? Assume volumes are additive.
214 mL
528 mL
1,214 mL
1,528 mL
7) What volume of a 100× stock solution is needed to prepare 500 mL of a 5× solution?
5 mL
10 mL
25 mL
50 mL
8) What is the formula for calculating a percent w/v solution?
(solute mass / solution mass) × 100
(solute mass / solution volume) × 100
(solution volume / solute mass) × 100
(solution volume / solute volume) × 100
9) What is the formula for calculating a percent w/w solution?
(solute mass / solution mass ) × 100
(solute mass / solution volume) × 100
(solution mass / solute mass) × 100
(solution mass / solute volume) × 100
10) What is the usual way to express solution concentration for a liquid solute dissolved in a liquid solvent?
%(m/m)
%(m/v)
%(v/m)
%(v/v)
11) If 0.5 mL of a sample is added to 10 mL of diluent, what is the dilution factor?
10
11
20
21
12) What is the salt concentration of a 500 mL solution containing 4.5 g of salt?
0.9 g/L
8 g/L
8.9 g/L
9 g/L
13) A dilution of 1/10 means what part specimen and what part diluent?
1 part specimen, 9 parts diluent
1 part specimen, 10 parts diluent
1 part specimen, 10.5 parts diluent
1 part specimen, 11 parts diluent
14) How much concentrate and water are needed to make 500 mL of buffer made from 2 parts concentrate and 6 parts water?
125 mL of concentrate and 375 mL of water
150 mL of concentrate and 350 mL of water
175 mL of concentrate and 325 mL of water
200 mL of concentrate and 300 mL of water
15) How many grams of sodium chloride are needed to make 200 mL of a 5% m/v sodium chloride solution?
5 g
10 g
15 g
20 g
16) A 200 mL solution is prepared by diluting 100 mL of ethanol with water. What is the % v/v concentration of the solution?
1%
10%
25%
50%
17) A technologist is preparing a serial dilution with eight tubes. The first tube contains a 1/2 dilution. The technologist dilutes each following tube to half the concentration of the previous tube. What is the dilution in the eighth tube?
1/128
1/256
1/512
1/1024
18) What is a molar solution?
1 gram of solute in 1 mL of solution
1 gram of solute in 1 mole of solution
1 mL of solute in 1 mole of solution
1 mole of solute in 1 liter of solution
19) Assuming volumes are additive, how much solute is needed to prepare 950 mL of a solution made from 7 parts solute and 10 parts solvent?
58 mL
101 mL
391 mL
559 mL
20) Which of these is the responsibility of a laboratory assistant?
Giving results to patients
Performing tests that require judgment
Preparing chemical solutions
Supervising other workers
21) A coworker asks you about a patient’s biopsy result, but they are not involved in the patient’s care. What should you do?
Refer the worker to the patient’s primary physician
Refuse to discuss the patient’s information
Share the result but omit the patient's name
Share the result only if the worker signs a confidentiality report
22) A technologist notices that an unauthorized staff member has been accessing patient records. What should she do?
Contact the patients involved
Delete the records immediately
Monitor the staff member's activity for a week to gather evidence
Report it to a supervisor
23) A technologist talks about a patient’s biopsy result in the hospital cafeteria. Which professional expectation is not being met?
Adaptability
Confidentiality
Cultural humility
Time management
24) Which of the following should be included in an employee's personnel file?
Most recent vacation destination
Performance reviews
Political affiliation
Web addresses of the employee's social media profiles
25) A technologist calls the ER department and says, "I have a critical hemoglobin of 5 g/dL for John Doe, MRN 12345." If the laboratory has a readback policy, what must the nurse reply?
"Please hold while I notify the physician immediately.”
"Please read the result back to me."
"That is a hemoglobin of 5 g/dL for John Doe, MRN 12345."
“Okay, that is a critical hemoglobin for John Doe, MRN 12345.”
26) A breast biopsy container is labelled "left breast", but the request form says "right breast". What should the laboratory do?
Contact the patient to confirm the correct side
Contact the surgical department
Discard the biopsy and request a new one
Process the specimen, but add a note in the file
27) Your neighbour asks about a friend’s biopsy result because he wants to know if it is serious. What should you do?
Call the patient to ask for permission
Give only general information
Say that you cannot discuss patient information
Tell your neighbour whether the result is benign or malignant
28) A technologist finds a bottle of stain reagent without a label. What should the technologist do?
Discard it down the sink
Treat it as hazardous
Use it if the color looks normal
Use it only on non-urgent specimens
29) A histology specimen is marked STAT, but its label does not match the request form. What should you do?
Ask the operating room to resolve the mismatch
Change the label to match the request form
Change the request form to match the label
Process it immediately
30) A patient’s family member asks a technologist for the patient's biopsy results. What should the technologist do?
Ask the family member to sign a Release of Information form
Give the result to the family member
Refer the family member to the pathologist
Refuse to release results without proper authorization
31) A technologist completes a stool culture but forgets to enter the result into the LIS. What is the main problem?
The LIS will delete the record
The bacteria will become resistant to antibiotics
The healthcare team may not receive the result
The result becomes automatically invalid
32) A technologist discovers that a culture plate was incubated at the wrong temperature overnight. What is the most appropriate action?
Ignore it if colonies are visible
Inform the supervisor and document the issue
Re-incubate the plate at the correct temperature
Report all results as preliminary
33) A technologist notices that a final report was released with an incorrect organism name. What should the technologist do?
Correct the report and notify appropriate personnel
Delete the incorrect report from the system
Do nothing because the report was already released
Request a new patient sample and notify the patient
34) A CSF Gram stain shows gram-negative diplococci. What should the laboratory do?
Report as normal flora
Report the result as a critical value
Request a new sample
Wait until full identification before reporting
35) A patient has the following lipid panel results.
Test
Result
Total cholesterol
160 mg/dL
LDL
100 mg/dL
HDL
40 mg/dL
Calculate the total cholesterol-to-HDL cholesterol ratio.
0.25
4
50
150
36) A patient's cholesterol results are as follows.
Test
Result
Triglycerides
200 mg/dL
Cholesterol
300 mg/dL
HDL
70 mg/dL
Use the Friedewald equation to calculate the patient's LDL.
190 mg/dL
200 mg/dL
210 mg/dL
220 mg/dL
37) Which of these chemistry results fails the CLIA quality requirements for proficiency testing?
To ensure employees understand how to use equipment
To prevent laboratory-acquired infections
To protect workers from harm
76) What should a laboratory do if it receives proficiency testing samples from another laboratory for testing?
Forward the samples to an outside laboratory
Notify the inspecting authority
Test the samples immediately
Test the samples the same way you test patient specimens
77) Which of these best meets the definition of proficiency testing?
Inspections to prevent work-related accidents
Testing for illegal drugs in a biological sample
Tests to ensure workers have the skills and knowledge to perform their jobs
The testing of unknown samples sent to the laboratory
78) A proficiency testing sample arrives during a busy shift. What should the technologist do with the specimen?
Give it to the most experienced technologist
Process it the same way as patient specimens
Test it first, before other specimens
Test it last, after all other work is complete
79) What is the purpose of external quality assessment?
To calibrate new laboratory instruments
To evaluate a laboratory's performance
To replace the need for daily controls
To track the expiration dates of reagents
80) What is the purpose of proficiency testing?
To evaluate a laboratory's testing accuracy
To monitor the rate of equipment malfunctions
To track the inventory levels of laboratory reagents
To train new employees on laboratory techniques
81) The quality control chart of an instrument shows ten consecutive data points on one side of the mean. What should you do?
Accept the run
Reject the run and follow the method's troubleshooting procedures
Repeat the control sera on the next run
Report the results with a disclaimer that they may be erroneous
82) In a control run, five consecutive measurements fall between the mean and +1 SD. What should the lab technician do?
Accept the control run
Accept the control run, but make a note of the incident in the log file
Reject the control run and take corrective action
Rerun the control run
83) In a run of twenty control samples, a single control value falls between 2 and 3 standard deviations from the mean. The other control readings are within the normal range, and no source of error can be identified. What is the most appropriate action to take?
Inform the clinician that results are likely to be incorrect
Prepare a new reagent
Replace the equipment
Report patient results
84) A laboratory accidentally releases a report with an incorrect blood group result. What should the laboratory do?
Amend the report and notify the clinical staff
Delete the original report and issue a new report
Request a new sample and issue a new report
Retest the original sample and issue a new report
85) During pretransfusion testing, the patient's ABO blood group does not match the historical record. What is the most appropriate action?
Change the historical record to match the new result
Report the current ABO result as the patient's blood group
Report the historical ABO result as the patient's blood group
Resolve the discrepancy
86) A laboratory switches from using a highly toxic chemical to a less toxic alternative. Which safety principle has the laboratory applied?
Administrative controls
Elimination
Engineering controls
Substitution
87) An AHG reagent fails daily QC, so the technologist opens a new vial, which passes QC. However, the technologist only documents the passed QC, not the failed QC. Why is this a problem?
All results will be legally invalid
The new vial is now contaminated
The test is now inaccurate
There is no record of the failure
88) Below is a blood bank control chart for anti-D reagent QC:
Day
Reaction strength
1
4+
2
4+
3
2+
4
4+
5
4+
6
4+
What does this pattern most likely suggest?
Random error
Shift
Systematic error
Trend
89) Which action could constitute battery?
Disclosing a patient's health information without consent
Holding down a patient to draw their blood against their will
Refusing to draw blood from a patient who arrives late to the appointment
Spreading malicious rumours about a colleague
90) A patient forgets to collect one urine sample during a 24-hour urine collection. What should usually happen?
Add extra urine
Estimate the volume of the missed sample
Restart the 24-hour collection
Submit the container and write “one sample missed” on the requisition
91) A courier delivers a chain of custody sample to a technician at a laboratory. Who needs to sign and date the chain of custody form?
Only the courier
Only the laboratory technician
The courier and the laboratory technician
The laboratory manager and the laboratory technician
92) Which of the following is NOT required on a chain of custody form?
The name of the patient's doctor
The name of the person who collected the specimen
The time the specimen was collected
The type of specimen collected
93) What should the laboratory do if a chain-of-custody form is missing?
Ask the courier to complete a new form
Fill out a new form yourself
Reject the specimen
Test the specimen, but add a comment
94) Which problem with a chain-of-custody form would automatically result in rejection of the specimen?
The courier's name is missing
The specimen ID does not match the ID on the form
The specimen temperature box is unticked
There is a digit error in the patient's ID number
95) Which of these tests requires chain of custody documentation?
Complete blood count
Fetal fibronectin
Free light chains test
Legal paternity test
96) A urine specimen for a drug screen arrives in the laboratory. However, the seal of the
specimen bottle is broken. What should you do?
Ask the courier to reseal the bottle
Reject the specimen
Test the specimen, but add a comment
Transfer the urine to a new, sterile container
97) A blood sample is unlabeled, but a nurse states that she knows which patient it belongs to. What is the best action?
Label it using the nurse’s information
Let the nurse label it herself
Reject it and request a new sample
Test it, but with a comment about the missing label
98) A blood sample arrives at the laboratory, but the name on the tube does not match the requisition. What should the laboratory do?
Accept the sample, but write a note in the LIS
Ask the phlebotomist to correct the label
Correct the label
Request a new sample
99) Which of these is a reason for rejecting a CBC specimen?
The patient was fasting
The specimen is at room temperature
The specimen is clotted
The specimen was collected in a lavender tube
100) A glucose specimen was left unprocessed at room temperature and discovered 3 hours later. What is the best action?
Add a glycolysis inhibitor and run the sample
Centrifuge the specimen and run the sample
Request a new sample
Shake the tube and run the sample
101) A laboratory fails to notify the doctor of a critical result. What type of error is this?
Analytical
Post-analytical
Pre-analytical
Quality control failure
102) Which of these is required when documenting a critical result communication?
The name of the laboratory director
The patient's date of birth
The patient’s room number
The person notified
103) A technologist notices that a critical result was entered into the LIS but not phoned, even though the SOP requires phone notification. What should the technologist do?
Delete the result
Document that the result is in the LIS
Nothing, because the result is already in the computer
Phone the result and document the notification
104) A lab technologist amends a report after an error is found. What should the technologist document?
Only the corrected result
The correction, department, laboratory telephone number, and person who made the change
The correction, patient's ID number, and technologist's initials
The correction, reason for amendment, date, time, and person who made the change
105) A laboratory audit reveals frequent delays in critical result reporting. Which intervention would most effectively solve this problem?
Eliminating verbal communication
Implementing escalation protocols
Increasing documentation requirements
Retraining staff on sample collection techniques
106) A laboratory issues blood for transfusion, but then discovers a mistake in the blood typing results. What should the laboratory do first?
Contact the clinical area
Delete the issue record and create a new record
Repeat testing on the original sample
Request a new sample
107) A technologist accidentally enters a susceptibility result under the wrong patient record. What is the most important concern?
The laboratory’s turnaround time will increase
The microorganisms could die before susceptibility testing occurs
The report may be delayed
The wrong patient may receive inappropriate treatment
108) You phone a frozen section diagnosis to the operating room. What is the most important action before ending the call?
Ask the receiver to read back the result
Confirm the patient's preferred pronouns
Document the call in the laboratory information system
Politely thank the receiver
109) According to OSHA, which vaccine must be offered to employees at risk of exposure to bloodborne pathogens?
HIV
Hepatitis A
Hepatitis B
MMR
110) What is the most effective type of hazard control in the ISO 45001 Hierarchy of Controls?
Administrative controls
Elimination
Engineering controls
Substitution
111) Which danger must employers inform workers about under OSHA’s Hazard Communication Standard?
Bloodborne pathogens
Dangerous chemicals
General safety hazards
Miscommunication
112) Which GHS pictogram is not mandatory under OSHA?
Environment
Exclamation mark
Flame over circle
Gas cylinder
113) In OSHA, a(n) __________ person is defined as "one who is capable of identifying existing and predictable hazards in the surroundings or working conditions which are unsanitary, hazardous, or dangerous to employees, and who has the authorization to take prompt corrective measures to eliminate them."
authorized
capable
competent
qualified
114) What type of hazard are OSHA action levels for?
Chemicals and noise
Ergonomic hazards
Psychosocial hazards
Slips, trips, and falls
115) What is the least effective type of hazard control in the ISO 45001 Hierarchy of Controls?
Administrative controls
Engineering controls
Personal protective equipment
Substitution
116) What is an exempt employee?
An employee who does not have to pay income taxes
An employee who is exempt from overtime rules
An employee working on a temporary or seasonal basis
An employee working remotely
117) Title I, Employment, is part of which civil rights law prohibiting discrimination?
Americans with Disabilities Act (ADA)
Civil Rights Act
Equal Employment Opportunity Act (EEOA)
Occupational Safety and Health Administration (OSHA)
118) Which federal law, passed in 1972, amended Title VII of the Civil Rights Act of 1964 to strengthen protections against employment discrimination?
Employment Non-Discrimination Act (ENDA)
Equal Employment Opportunity Act (EEOA)
Equal Pay Act
Rehabilitation Act
119) Which act regulates whether employees are exempt or nonexempt?
Civil Rights Acts
Equal Pay Act (EPA)
Fair Labor Standards Act (FLSA)
Occupational Safety and Health Act (OSHA)
120) How many weeks of unpaid leave does the Family and Medical Leave Act (FMLA) allow employees within a 12-month period without jeopardizing their job?
12 weeks
20 weeks
24 weeks
36 weeks
121) What legal term describes a person who is not of sound mind and is considered legally incapable of responsibility for their actions?
Corpus juris
Habeas corpus
Non compos mentis
Res ipsa loquitur
122) Which legal term means the degree of care a prudent, reasonable healthcare provider would exercise in a given situation?
Customary care
Optimal care
Scope of practice
Standard of care
123) What could a physician be held liable for if he fails to inform the patient of the risks and benefits of a procedure?
Assault
Battery
Fraud
Negligence
124) What federal agency regulates the manufacturing of prescription medications?
American Dental Association (ADA)
Drug Enforcement Administration (DEA)
Food and Drug Administration (FDA)
Occupational Safety and Health Administration (OSHA)
125) What federal agency administers and enforces the Occupational Safety and Health Act?
Environmental Protection Agency (EPA)
National Institute for Occupational Safety and Health (NIOSH)
Occupational Safety and Health Administration (OSHA)
United States Public Health Service (USPHS)
126) Which federal agency regulates the transport of hazardous materials?
Centers for Disease Control and Prevention (CDC)
Department of Transportation (DOT)
Food and Drug Administration (FDA)
National Highway Traffic Safety Administration (NHTSA)
127) Which federal agency regulates clinical laboratories through the CLIA program?
Center for Global Health (CGH)
Centers for Medicare and Medicaid Services (CMS)
Health Resources and Services Administration (HRSA)
Office of Public Health Science (OPHS)
128) Which agency enforces the HIPAA Privacy and Security Rules?
The Attorney General
The Office for Civil Rights
The Surgeon General
The US Senate
129) Which agency provides procedures and reagents for rapidly detecting biothreats and emerging infectious diseases?
Centers for Disease Control and Prevention (CDC)
Centers for Medicare & Medicaid Services (CMS)
Environmental Protection Agency (EPA)
Occupational Safety and Health Administration (OSHA)
130) Which federal agency regulates in vitro diagnostic tests?
Federal Laboratory Consortium (FLC)
Food and Drug Administration (FDA)
National Institutes of Health (NIH)
Occupational Safety and Health Administration (OSHA)
131) Which federal agency investigates complaints of job discrimination?
Equal Employment Opportunity Commission (EEOC)
Fair Labor Association (FLA)
Food and Drug Administration (FDA)
Occupational Safety and Health Administration (OSHA)
132) Which federal agency sets the standards and guidelines for Hospital, Medical, and Infectious Waste Incinerators (HMIWI)?
Centers for Disease Control and Prevention (CDC)
Environmental Protection Agency (EPA)
Food and Drug Administration (FDA)
Occupational Safety and Health Administration (OSHA)
133) Which federal agency requires employers to provide safety training to their employees?
National Institute for Occupational Safety and Health (NIOSH)
Occupational Safety and Health Administration (OSHA)
United States Department of the Interior (USDI)
United States Office of Personnel Management (USOPM)
134) Which federal agency enforces workplace safety laws?
Food and Drug Administration (FDA)
National Institutes of Health (NIH)
Occupational Safety and Health Administration (OSHA)
Office of Public Health Science (OPHS)
135) Which federal agency regulates commercially marketed in vitro diagnostic tests?
American Clinical Laboratory Association (ACLA)
Clinical Laboratory Improvement Amendments (CLIA)
Food and Drug Administration (FDA)
Occupational Safety and Health Administration (OSHA)
136) Which federal agency requires employers to provide workers with PPE?
Centers for Disease Control and Prevention (CDC)
National Institute for Occupational Safety and Health (NIOSH)
National Institutes of Health (NIH)
Occupational Safety and Health Administration (OSHA)
137) What federal agency categorizes clinical laboratory tests by their complexity?
Centers for Disease Control and Prevention (CDC)
Clinical Laboratory Improvement Amendments (CLIA)
Food and Drug Administration (FDA)
Occupational Safety and Health Administration (OSHA)
138) What federal agency regulates general-purpose surface disinfectants?
American Medical Association (AMA)
Drug Enforcement Administration (DEA)
Environmental Protection Agency (EPA)
Food and Drug Administration (FDA)
139) Which federal agency enforces federal employment discrimination laws, such as the Civil Rights Act and the Americans with Disabilities Act (ADA)?
Employee Benefits Security Administration (EBSA)
Employment and Training Administration (ETA)
Equal Employment Opportunity Commission (EEOC)
Occupational Safety and Health Administration (OSHA)
140) Which federal agency regulates laboratory testing performed on humans?
American Medical Association (AMA)
Centers for Disease Control and Prevention (CDC)
Centers for Medicare & Medicaid Services (CMS)
Food and Drug Administration (FDA)
141) Which law sets the federal minimum wage?
Civil Rights Act
Equal Pay Act
Fair Labor Standards Act (FLSA)
National Labor Relations Act (NLRA)
142) Which federal law prohibits employment discrimination based on race, color, religion, sex, and national origin?
Americans with Disabilities Act
Civil Rights Act
Fair Labor Standards Act (FLSA)
National Labor Relations Act (NLRA)
143) Which law requires laboratories to document the competency of their personnel?
Clinical Laboratory Improvement Amendments (CLIA)
Fair Labor Standards Act (FLSA)
National Labor Relations Act (NLRA)
Occupational Safety and Health Act (OSHA)
144) Which law requires healthcare providers to ensure effective communication with deaf patients, such as providing a qualified interpreter?
Americans with Disabilities Act (ADA)
Family and Medical Leave Act (FMLA)
Health Care Right of Conscience Act (HCRCA)
Health Insurance Portability and Accountability Act (HIPAA)
145) Which act outlines the lawful use and disclosure of protected health information?
Digital Accountability and Transparency Act (DATA)
Freedom of Information Act (FOIA)
Health Insurance Portability and Accountability Act (HIPAA)
Privacy Act
146) Which act gives workers the right to know about hazards in the workplace?
Health Care Consent Act (HCCA)
Occupational Safety and Health Act
Privacy Act
SAFETY Act
147) Which law requires safeguards when sending health information by email?
CAN-SPAM Act
Health Insurance Portability and Accountability Act (HIPAA)
Occupational Safety and Health Act (OSHA)
Privacy Act
148) Which OSHA standard did the Needlestick Safety and Prevention Act amend?
Bloodborne Pathogens Standard
Confined Space Entry Standard
Hazard Communication Standard
Personal Protective Equipment Standard
149) Which act prevents employers from retaliating against workers for complaining about unsafe working conditions?
Fair Labor Standards Act (FLSA)
Federal Employees' Compensation Act (FECA)
Occupational Safety and Health Act (OSHA)
Uniformed Services Employment and Reemployment Rights Act (USERRA)
150) Under the American National Standards Institute (ANSI) standards, which safety sign indicates a potential hazard that may result in minor or moderate injury?
Caution
Danger
Notice
Warning
151) The Globally Harmonized System of Classification and Labeling of Chemicals has two signal words for hazardous chemicals: "Danger" and:
Attention
Caution
Notice
Warning
152) How often does CLIA require routine inspections of non-waived laboratories?
Every three months
Every six months
Every year
Every two years
153) According to CLIA, who is responsible for the day-to-day supervision of the laboratory operation and its personnel?
General supervisor
Laboratory director
Technical supervisor
Testing personnel
154) How often does CLIA require calibration verification of test systems?
Every three months
Every six months
Every year
Every two years
155) When are laboratories exempt from proficiency testing?
When they are CLIA certified
When they have a Certificate of Compliance
When they have six or fewer employees
When they only perform waived tests
156) Under CLIA regulations, which of these is a high-complexity test?
Complete blood count
Drugs of abuse testing
Electrolyte profile
Examination of a blood smear
157) According to CLIA, who is responsible for specimen processing, test performance, and reporting test results?
Laboratory director
Technical consultant
Technical supervisor
Testing personnel
158) Which CLIA certificate allows laboratories to conduct moderate or high complexity tests before the laboratory is inspected and a final certificate is issued?
Accreditation
Compliance
Registration
Waiver
159) Under CLIA regulations, how many categories of lab test complexity are there?
3
5
6
8
160) What does a Certificate of Waiver allow a facility to do?
Conduct tests without patient consent
Perform simple, low-risk laboratory tests
Store controlled substances without a DEA license
Use RoHS-exempt equipment
161) According to CLIA, who is responsible for the technical and scientific oversight of the laboratory?
Laboratory technician
Medical equipment repairer
Technical consultant
Test system manufacturer
162) CLIA refers to moderate and high complexity tests collectively as _____________ tests.
cleared
licensed
nonwaived
point-of-care
163) Which of these tests is most likely to be CLIA-waived?
Complete blood count
Immunoassay
Peripheral smear
Urine hCG test
164) According to CLIA, who is responsible for the overall operation and administration of the laboratory?
Clinical consultant
Laboratory director
Technical consultant
Technical supervisor
165) When should laboratories use alternative assessment?
For highly complex tests
For tests that have critical values
When a traditional proficiency test is not available
When the laboratory is not yet CLIA-certified
166) According to CLIA, how many areas of competency must be included in a laboratory competency assessment program?
6
7
8
9
167) Which CLIA category of test complexity does dipstick urinalysis fall under?
High complexity
Moderate complexity
Provider performed microscopy
Waived
168) What is mixed with a weak acid or a weak base to create a buffer?
A salt solution
A strong acid or strong base
Acetic acid
The conjugate salt
169) How many times more acidic is pH 5 than pH 6?
1
2
10
100
170) Which term describes a substance that minimizes changes in pH?
Anhydride
Buffer
Compound
Solvent
171) What is the term for a solution with a pH value of less than 7?
Acidic
Alkaline
Basic
Neutral
172) Which ion does pH measure?
Carbon
Hydrogen
Nitrogen
Oxygen
173) Which pH is neutral?
0
1
3
7
174) For data distributed normally, what percentage of values fall within one standard deviation of the mean?
50%
68%
83%
95%
175) In a normal distribution, what percentage of cases are above the mean?
50%
68%
75%
100%
176) The following data were calculated on a series of 100 determinations of a control.
Mean
5.8
Median
6.0
Standard deviation
0.25
If control limits are set at ±2 standard deviations, what are the allowable limits for the control?
5.30–6.30
5.35–6.25
5.50–5.90
5.60–5.90
177) For data distributed normally, what percentage of values fall within two standard deviations of the mean?
50%
68%
83%
95%
178) If a data set has a mean of 7.2 and a standard deviation of 1.2, what range of values falls within two standard deviations of the mean?
4.8–9.6
6.0–8.4
7.2–9.6
8.6–10.2
179) For data distributed normally, what percentage of values fall within three standard deviations of the mean?
68%
83%
95%
99.7%
180) Assuming values are normally distributed, what range should encompass 95% of observed values if a control has a mean of 5 and a standard deviation of 1?
1–9
2–8
3–7
4–6
181) Which formula calculates the coefficient of variation?
(Mean / standard deviation) × 100
(Standard deviation / mean) × 100
(Standard deviation / variance) × 100
(Variance / mean) × 100
182) Automated white blood cell counts are performed on 29 blood samples. The mean value is 8.0 cells/nL, and the standard deviation is 1.2 cells/nL. What is the coefficient of variation?
15%
30%
45%
60%
183) If the mean is 20 and two standard deviations is 8, what is the coefficient of variation?
0.2%
4%
20%
40%
184) Ten patients have the following bilirubin results: 3.1, 3.2, 3.8, 4.1, 4.7, 5.1, 5.9, 6.0, 6.2, 6.9. Calculate the mean of these results.
4.5
4.7
4.9
5.1
185) What is the term for the middle value in a sorted data set?
Average
Coefficient of variation
Mean
Median
186) What is the term for the value that appears most often in a set of data values?
Average
Coefficient of variation
Mean
Mode
187) Where should bulk quantities of acetone be kept?
Biological safety cabinet
Flammable storage cabinet
Freezer
Refrigerator
188) How is diethyl ether stored?
In airtight containers
In the light
In the refrigerator
Underwater
189) Where should stock ethanol solutions be stored?
Flammable safety cabinet
Freezer
Fume hood
Refrigerator
190) Under OSHA's Hazard Communication Standard, which of the following information is mandatory on hazardous chemical labels?
Environmental pictogram
Expiration date
Personal protective equipment (PPE) pictogram
Phone number of the manufacturer or importer
191) If a chemical has a hazard that warrants a “Danger” signal word and another hazard that warrants the “Warning” signal word, then what signal word should appear on the label?
"Fatal"
Both "Warning" and "Danger"
Only the “Danger” signal word
Only the “Warning” signal word
192) A small acetone spill occurs on the bench near a lit Bunsen burner. What should be the first action?
Clean up the spill
Move everyone to fresh air
Turn off the flame
Wash eyes with water
193) Which of these substances would be the best solution to clean up a large blood spill?
1:10 dilution of bleach
70% isopropyl alcohol
Antibacterial soap and water
Povidone-iodine
194) Which concentration of bleach solution is used to decontaminate benchtops?
10%
20%
30%
50%
195) What does cold sterilization use to sterilize medical instruments?
Chemicals
Cold air
Ice
Liquid nitrogen
196) What is the first step when cleaning up a blood spill?
Pour disinfectant onto the spill
Put on PPE
Record the incident in the incident book
Wash your hands
197) Which of these ethyl alcohol dilutions is the most effective disinfectant?
10% ethyl alcohol
40% ethyl alcohol
70% ethyl alcohol
100% ethyl alcohol
198) Which chemical is used to decontaminate equipment exposed to prions?
Alcohol
Formaldehyde
Hydrogen peroxide
Sodium hydroxide
199) Which cleaning product releases a toxic gas when used to clean urine spills?
Chlorine bleach
Hydrogen peroxide
Isopropyl alcohol
Povidone-iodine
200) Which chemical is best for neutralizing weak acid spills?
Acetic acid
Boric acid
Sodium bicarbonate
Sodium hydroxide
201) Mercury spills are most dangerous when the spill occurs:
in a fume hood
in a poorly ventilated area
near sources of ignition
near the flammable storage cabinet
202) Which of these is used for cleaning minor liquid radioactive spills?
Bleach
Lead sheet
Paper towels
Soda ash
203) What pressure and temperature are commonly used for steam sterilization in an autoclave?
12 psi at 115°C
12 psi at 120°C
15 psi at 112°C
15 psi at 121°C
204) Which type of glass is autoclavable?
Borosilicate
Float
Lead
Soda-lime
205) Which process kills all microorganisms, including spores?
Cleaning
Disinfection
Sanitization
Sterilization
206) Why should a dry-heat sterilizer remain closed throughout the sterilization cycle?
To avoid damaging the oven
To ensure complete sterilization
To prevent moisture from entering the chamber
To prevent oxygen from entering the chamber
207) Which of these is a Standard Precaution?
Fire extinguisher
Hand hygiene
Keeping chemical containers closed
Radiation shielding
208) What is the meaning of a 4 in the yellow quadrant of an NFPA hazard diamond?
The substance is highly radioactive
The substance is extremely contagious
The substance readily burns at normal temperatures
The substance readily explodes
209) Which color on the NFPA hazard diamond signals the degree of hazards to health?
Blue
Green
White
Yellow
210) What is the meaning of a 1 in the red quadrant on the NFPA hazard diamond?
The substance does not burn
The substance only ignites after considerable preheating
The substance ignites if moderately heated
The substance can be ignited at normal temperatures
211) On the NFPA hazard diamond below, what do the letters SA mean?
Can shock or cause acid damage
Safety assessed
Simple asphyxiant
Substance abuse
212) What does a number 2 in the blue quadrant of an NFPA hazard diamond indicate?
Continued exposure to the substance could cause temporary incapacitation or injury
The substance can be ignited at normal temperatures
The substance is unreactive with water
The substance reacts with water in an unusual or dangerous way
213) In the NFPA hazard diamond, which quadrant is for special hazards?
Blue
Red
White
Yellow
214) Which prefix means 10⁻⁶?
Centi-
Kilo-
Micro-
Nano-
215) What does the prefix kilo- mean?
10
100
1,000
10,000
216) What does the prefix nano- mean?
1 hundredth
1 thousandth
1 millionth
1 billionth
217) What does the symbol μ represent in SI units?
10⁹
10⁻³
10⁻⁶
10⁻⁹
218) What are cubic decimeters more commonly known as?
Grams
Liters
Meters cubed
Moles
219) Which prefix means 0.001?
Micro-
Centi-
Pico-
Milli-
220) What is 0.05 mL in microliters?
0.0005 μL
0.005 μL
5 μL
50 μL
221) What is the following graph shape?
Normal distribution
Polynomial function
Quadratic chart
Reciprocal graph
222) Which federal agency regulates the disposal of hazardous waste produced by laboratories?
Centers for Disease Control and Prevention (CDC)
Centers for Medicare & Medicaid Services (CMS)
Environmental Protection Agency (EPA)
Food and Drug Administration (FDA)
223) Which act regulates hazardous waste disposal?
Clean Water Act (CWA)
National Environmental Policy Act (NEPA)
Pollution Prevention Act (PPA)
Resource Conservation and Recovery Act (RCRA)
224) Which of these is regulated waste?
Empty medicine bottles
Gauze packaging
Sterile dressings
Used blood tubes
225) Where should you discard used needles?
Biohazard sharps container
Glass disposal bucket
Glass recycling bin
Regular garbage
226) What is an upper flammability limit?
The amount of fuel that will burn before the fuel burns itself out
The highest concentration of a vapor that can catch fire
The highest temperature at which a liquid can be stored safely
The highest temperature that a chemical can reach when ignited
227) Under OSHA regulations, a flammable liquid has a flash point at or below which temperature?
37.8°C
67.3°C
93°C
100°C
228) Refer to the image below.Which of these fires can this fire extinguisher extinguish?
Cardboard boxes
Electrical equipment
Methanol
Oil bath
229) A fire extinguisher has the following symbol.This fire extinguisher:
can be used on fires involving flammable liquids or gases
requires annual top-ups of type B fuel
should be stored in a separate room from flammable chemicals
uses kerosene to extinguish fires
230) What are the three components of the fire triangle?
Oxygen, heat, and fuel
Oxygen, ignition, and fuel
Rescue, Alarm, Escape
Rescue, Alarm, Extinguish
231) What does the acronym RACE mean in the context of fire procedures?
Ready, Aim, Check, Extinguish
Recover, Act, Control, Extinguish/Evacuate
Rescue, Alarm, Confine, Extinguish/Evacuate
Run, Activate, Coordinate, Extinguish/Evacuate
232) A fire extinguisher has the following symbol:This fire extinguisher:
can extinguish electrical fires
cannot be used on electrical equipment
carries a risk of electric shock
requires charging before use
233) Which of these chemicals is flammable?
Acetone
Carbon tetrachloride
Chloroform
Saline
234) Where should paraformaldehyde solutions be prepared?
In a biological safety cabinet
In a chemical fume hood
In the sink
Under a laminar flow clean bench
235) A 500 mL bottle of 35% formaldehyde falls on the floor and breaks. What should be your first action?
Dilute the spill with water
Evacuate the area
Put on PPE
Record the incident in the incident log
236) At low concentrations, what does hydrogen sulfide smell like?
Burnt sugar
Rotten eggs
Rotting meat
Strong cheese
237) When mixing acid and water:
add the acid quickly to the water
add the acid slowly to the water
add the water quickly to the acid
add the water slowly to the acid
238) Where should you handle chemicals that produce harmful vapors?
Autoclave
Biological safety cabinet
Fume hood
Sealed room
239) What should be your first action in the event of an acid burn?
Cover the burn with gauze moistened in sterile saline
Flush the burn with water
Go to the emergency department
Neutralize the acid with sodium bicarbonate
240) What is the minimum amount of time the eyes should be flushed with water in the event of a chemical splash?
1 minute
5 minutes
10 minutes
15 minutes
241) A lab technologist has spilled concentrated hydrochloric acid onto his clothing and skin, affecting a large portion of his body. After he has removed his clothing, what is the next thing he should do?
Apply a burn ointment to his skin
Pour baking soda onto his skin
Seek emergency medical assistance
Use the emergency safety shower
242) Which type of hazard do universal precautions apply to?
Bloodborne pathogens
Dangerous chemicals
Environmental hazards
Radiation
243) Which biosafety level does a nonpathogenic strain of E. coli require?
BSL-1
BSL-2
BSL-3
BSL-4
244) Which of these is an OPIM (other potentially infectious material)?
Blood
Semen
Urine
Vomit
245) The responsibility for identifying employees at risk of exposure to blood or OPIM lies with the:
employer
government
patient
union
246) What is the pictogram for a pressurized gas?
A flame
A flame over a circle
A gas cylinder
An exploding test tube
247) Which of these is found on safety data sheets?
Expiry date
Health hazards
Price
Production date
248) How many sections are in a standard GHS safety data sheet?
8
10
12
16
249) Which centrifuge is used to spin capillary tubes?
Angle head
Cytospin
Microhematocrit
Refrigerated
250) Which centrifuge concentrates cells from a fluid specimen onto a microscope slide for morphological examination?
Angle head
Cytocentrifuge
Microhematocrit
Refrigerated
251) What should you do before turning on a centrifuge?
Ensure the centrifuge is balanced
Open the cover
Place the centrifuge on a slanted surface
Uncover all liquid specimen tubes
252) What does RPM indicate on a centrifuge?
How fast the centrifuge spins
How long the centrifuge spins for
How many tubes the centrifuge can hold
The maximum weight the rotor can safely handle
253) Which device measures the RPM of a centrifuge?
Ohmmeter
Rheostat
Tachometer
Voltmeter
254) What is the most common cause of excessive centrifuge vibration?
Electrical interference
Unbalanced tubes
Uneven bench surfaces
Variable voltage
255) What is the term for water from which charged particles have been removed?
Chlorinated water
De-ionized water
Distilled water
Flat water
256) What is the term for a solution in which no more solute can be dissolved?
Concentrated
Diluted
Saturated
Soluted
257) Which microscope is used most in clinical laboratories?
Acoustic
Electron
Light
Scanning probe
258) Which part of a microscope controls the brightness of the light bulb?
Diaphragm
Numerical aperture
Rheostat
Stage clip
259) Which part of a microscope controls how much light reaches the specimen?
Diaphragm
Numerical aperture
Objective lens
Rheostat
260) Which objective lenses do compound light microscopes usually have?
2×, 10×, 50×, and 100×
2×, 20×, 50×, and 80×
4×, 10×, 40×, and 100×
5×, 20×, 40×, and 80×
261) Which microscope is best for examining an unstained, wet preparation of cells before dehydration?
Brightfield
Electron
Fluorescence
Phase contrast
262) Which part of a microscope focuses light onto the specimen?
Condenser
Diaphragm
Objective lens
Ocular lens
263) Which magnification is typically used to examine the morphology of bacteria?
10x
100x
400x
1000x
264) You notice dirt in the field of view while using a microscope. The dirt moves when you rotate the eyepiece. What action should you take?
Clean the eyepiece
Clean the objective
Open the iris
Tighten the tension adjustment
265) A pipette gives a reading of 4.9 mL, but the true value of the volume of liquid inside the pipette is 5.0 mL. What is the percent error?
2%
4%
6%
8%
266) Which item of glassware is designed to deliver specific volumes?
Beaker
Erlenmeyer flask
Volumetric flask
Volumetric pipette
267) Which pipette is the most accurate?
Graduated
Mohr
Pasteur
Volumetric
268) What does "10 mL TD 25 SEC" on a pipette mean?
The pipette can be filled with 10 mL in 25 seconds
The pipette can deliver 10 mL in its primary tube and 25 mL in its secondary tube
The pipette is calibrated to deliver 10 mL after a 25-second drain time
You should wait 25 seconds between filling and dispensing the pipette
269) A to-deliver pipettor has an accuracy of +/- 0.8%. When using the pipette to deliver a volume of 100 µL, what range would you expect the volume of liquid to fall within?
99.05–100.95 µL
99.2–100.8 µL
99.55–100.45 µL
99.85–100.15 µL
270) What do the double rings mean on this pipette?
The last drop must be blown out
The pipette can hold 2 mL
The pipette will deliver the measured amount of liquid only at 20°C
The volume is measured at this point
271) Which pipette has a bulged-out portion in the middle?
Mohr
Pasteur
Serological
Volumetric
272) At which level should a volumetric pipette be viewed for an accurate reading?
At an angle
At eye level
Just above eye level
Just below eye level
273) Which glassware is used to measure 24-hour urine volumes?
Beaker
Erlenmeyer flask
Graduated cylinder
Graduated pipette
274) Which type of pipette is used to measure and dispense various volumes using calibration marks (graduations) along the entire length of the tube?
Disposable
Pasteur
Serological
Volumetric
275) What does a biological safety cabinet protect against?
Infectious aerosols
Thermal burn injuries
UV radiation
Volatile chemical vapors
276) How often should biological safety cabinets be certified?
Once a year
Every 2 years
Every 3 years
Every 4 years
277) What type of filter in biological safety cabinets removes airborne particles such as bacteria and viruses?
Electrostatic
Fiberglass
HEPA
UV light
278) Which grade of chemical is used in analytical laboratory work that requires high-purity chemicals?
AR
CP
LR
USP
279) Which hazard category do formalin, xylene, and other reagents fall into?
Biological
Chemical
Magnetic
Radiation
280) Which situation should be documented as a laboratory incident?
A culture plate is moved to the incubator
A specimen arrives leaking
A technician takes a scheduled 15-minute break
The LIS autoverifies results
281) Which of these actions reduces the risk of repetitive strain injury?
Repeating the same movement many times
Resting your arms on a hard surface
Sitting in the same position for a long period of time
Taking frequent breaks
282) What is the name of the process of assessing the severity and likelihood of workplace hazards?
Health needs evaluation
Myers-Briggs type indicator
Risk assessment
Workplace danger evaluation
283) What does the mnemonic PASS help to remember?
The key safety principles of working in a laboratory
The steps for first aid
The steps to operate a fire extinguisher
The warning signs of a stroke
284) What is the term for a document that identifies hazards and the ways to minimize their impact?
Accident and incident recording form
First aid assessment form
Inspection report
Risk assessment
285) What is the universal solvent?
0.85% sodium chloride
10% ethanol
8.5% sodium chloride
Water
286) Which of these describes preventive maintenance?
Identifying unexpected failures
Repairing equipment after it fails
Servicing equipment before it fails
Turning off machines when they are not in use
287) Which of these chemical grades has the highest purity?
Commercial
Laboratory
Reagent
Technical
288) Which equipment measures small masses with high precision?
Analytical balance
Desiccator
PH meter
Single pan balance
289) What is the function of a buffer?
To accentuate changes in pH
To make a solution more acidic
To make a solution more basic
To minimize changes in pH
290) Triple-beam balances give readings in what unit of measurement?
Grams
Kilograms
Liters
Milliliters
291) Which pH buffer solution is typically used first when calibrating a pH meter?
pH 1
pH 4
pH 7
pH 10
292) What substance is used to calibrate refractometers?
Buffer solution
Distilled water
Healthy urine
Hydrochloric acid
293) Which water purification method involves boiling water into steam, and then letting the steam cool?
Deionization
Distillation
Filtration
Reverse osmosis
294) How is relative centrifugal force (RCF) converted from revolutions per minute (RPM)?
RCF = RPM × 1.144 × 10⁵ × r²
RCF = RPM × 1.144 × 10⁻⁵ × r²
RCF = RPM² × 1.118 × 10⁵ × r
RCF = RPM² × 1.118 × 10⁻⁵ × r
295) Which of these best defines 'turnaround time'?
The duration of a blood cell count
The time from specimen receipt to result reporting
The time required for sample preparation
The time taken for instrument maintenance
296) Solid crystals of potassium oxalate are added to distilled water in a container. Which term describes the potassium oxalate?
Reagent
Solute
Solution
Solvent
297) What is a delta check?
A comparison of a laboratory's results to those from an outside facility
A comparison of current test results to previous results
An inspection to ensure all patients are wearing wristbands
The verification of a patient's identity using at least three identifiers
298) Which of these actions would increase a laboratory's efficiency?
Increasing the time it takes to perform key tasks
Keeping frequently used equipment within easy reach
Using an inconsistent labeling system
Using paper files instead of digital files
299) What is the term for a solution that contains less solute than the maximum amount of solute that can be dissolved?
Diluted
Saturated
Supersaturated
Unsaturated
300) A hypertonic solution:
causes cells to grow
contains a higher concentration of solutes than another solution
contains a lower concentration of solutes than another solution
contains the same concentration of solutes as another solution
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