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MT(AAB) Basic Knowledge practice questions

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1) A phlebotomist fails to check patient identifiers before collecting blood samples. What is the most likely consequence?
  1. Clotting
  2. Hemolysis
  3. Increased turnaround time
  4. Specimen misidentification
2) A patient arrives for a blood draw when the phlebotomist realizes she forgot to label the collection tubes from the previous patient, who has already left. What should the phlebotomist do?
  1. Discard the tubes and schedule a redraw
  2. Label the tubes immediately using the information from requisition form
  3. Send the unlabelled tubes to the laboratory with a note of explanation
  4. Set the tubes aside and label them later
3) A phlebotomist accidentally collects a coagulation test in a lavender-top tube instead of a light blue-top tube. What should the phlebotomist do?
  1. Add sodium citrate to the red-top tube
  2. Pour the blood sample into the correct tube
  3. Reject the specimen and recollect it in the correct tube
  4. Relabel the lavender-top tube as a light blue-top tube
4) A technologist notes a clot in an EDTA sample. What is the most appropriate action?
  1. Dilute the sample to account for the trapped cells
  2. Gently stir the sample to break up the clot
  3. Reject the specimen and request a new one
  4. Remove the clot with an applicator stick
5) You are labeling a specimen when a coworker interrupts you to ask a question. What is the best action?
  1. Answer your coworker's question first
  2. Ask someone else to label the specimen
  3. Label the specimen first
  4. Redraw the specimen
6) How much water must be added to 1 liter of 85% v/v alcohol to dilute it to 70%? Assume volumes are additive.
  1. 214 mL
  2. 528 mL
  3. 1,214 mL
  4. 1,528 mL
7) What volume of a 100× stock solution is needed to prepare 500 mL of a 5× solution?
  1. 5 mL
  2. 10 mL
  3. 25 mL
  4. 50 mL
8) What is the formula for calculating a percent w/v solution?
  1. (solute mass / solution mass) × 100
  2. (solute mass / solution volume) × 100
  3. (solution volume / solute mass) × 100
  4. (solution volume / solute volume) × 100
9) What is the formula for calculating a percent w/w solution?
  1. (solute mass / solution mass ) × 100
  2. (solute mass / solution volume) × 100
  3. (solution mass / solute mass) × 100
  4. (solution mass / solute volume) × 100
10) What is the usual way to express solution concentration for a liquid solute dissolved in a liquid solvent?
  1. %(m/m)
  2. %(m/v)
  3. %(v/m)
  4. %(v/v)
11) If 0.5 mL of a sample is added to 10 mL of diluent, what is the dilution factor?
  1. 10
  2. 11
  3. 20
  4. 21
12) What is the salt concentration of a 500 mL solution containing 4.5 g of salt?
  1. 0.9 g/L
  2. 8 g/L
  3. 8.9 g/L
  4. 9 g/L
13) A dilution of 1/10 means what part specimen and what part diluent?
  1. 1 part specimen, 9 parts diluent
  2. 1 part specimen, 10 parts diluent
  3. 1 part specimen, 10.5 parts diluent
  4. 1 part specimen, 11 parts diluent
14) How much concentrate and water are needed to make 500 mL of buffer made from 2 parts concentrate and 6 parts water?
  1. 125 mL of concentrate and 375 mL of water
  2. 150 mL of concentrate and 350 mL of water
  3. 175 mL of concentrate and 325 mL of water
  4. 200 mL of concentrate and 300 mL of water
15) How many grams of sodium chloride are needed to make 200 mL of a 5% m/v sodium chloride solution?
  1. 5 g
  2. 10 g
  3. 15 g
  4. 20 g
16) A 200 mL solution is prepared by diluting 100 mL of ethanol with water. What is the % v/v concentration of the solution?
  1. 1%
  2. 10%
  3. 25%
  4. 50%
17) A technologist is preparing a serial dilution with eight tubes. The first tube contains a 1/2 dilution. The technologist dilutes each following tube to half the concentration of the previous tube. What is the dilution in the eighth tube?
  1. 1/128
  2. 1/256
  3. 1/512
  4. 1/1024
18) What is a molar solution?
  1. 1 gram of solute in 1 mL of solution
  2. 1 gram of solute in 1 mole of solution
  3. 1 mL of solute in 1 mole of solution
  4. 1 mole of solute in 1 liter of solution
19) Assuming volumes are additive, how much solute is needed to prepare 950 mL of a solution made from 7 parts solute and 10 parts solvent?
  1. 58 mL
  2. 101 mL
  3. 391 mL
  4. 559 mL
20) Which of these is the responsibility of a laboratory assistant?
  1. Giving results to patients
  2. Performing tests that require judgment
  3. Preparing chemical solutions
  4. Supervising other workers
21) A coworker asks you about a patient’s biopsy result, but they are not involved in the patient’s care. What should you do?
  1. Refer the worker to the patient’s primary physician
  2. Refuse to discuss the patient’s information
  3. Share the result but omit the patient's name
  4. Share the result only if the worker signs a confidentiality report
22) A technologist notices that an unauthorized staff member has been accessing patient records. What should she do?
  1. Contact the patients involved
  2. Delete the records immediately
  3. Monitor the staff member's activity for a week to gather evidence
  4. Report it to a supervisor
23) A technologist talks about a patient’s biopsy result in the hospital cafeteria. Which professional expectation is not being met?
  1. Adaptability
  2. Confidentiality
  3. Cultural humility
  4. Time management
24) Which of the following should be included in an employee's personnel file?
  1. Most recent vacation destination
  2. Performance reviews
  3. Political affiliation
  4. Web addresses of the employee's social media profiles
25) A technologist calls the ER department and says, "I have a critical hemoglobin of 5 g/dL for John Doe, MRN 12345." If the laboratory has a readback policy, what must the nurse reply?
  1. "Please hold while I notify the physician immediately.”
  2. "Please read the result back to me."
  3. "That is a hemoglobin of 5 g/dL for John Doe, MRN 12345."
  4. “Okay, that is a critical hemoglobin for John Doe, MRN 12345.”
26) A breast biopsy container is labelled "left breast", but the request form says "right breast". What should the laboratory do?
  1. Contact the patient to confirm the correct side
  2. Contact the surgical department
  3. Discard the biopsy and request a new one
  4. Process the specimen, but add a note in the file
27) Your neighbour asks about a friend’s biopsy result because he wants to know if it is serious. What should you do?
  1. Call the patient to ask for permission
  2. Give only general information
  3. Say that you cannot discuss patient information
  4. Tell your neighbour whether the result is benign or malignant
28) A technologist finds a bottle of stain reagent without a label. What should the technologist do?
  1. Discard it down the sink
  2. Treat it as hazardous
  3. Use it if the color looks normal
  4. Use it only on non-urgent specimens
29) A histology specimen is marked STAT, but its label does not match the request form. What should you do?
  1. Ask the operating room to resolve the mismatch
  2. Change the label to match the request form
  3. Change the request form to match the label
  4. Process it immediately
30) A patient’s family member asks a technologist for the patient's biopsy results. What should the technologist do?
  1. Ask the family member to sign a Release of Information form
  2. Give the result to the family member
  3. Refer the family member to the pathologist
  4. Refuse to release results without proper authorization
31) A technologist completes a stool culture but forgets to enter the result into the LIS. What is the main problem?
  1. The LIS will delete the record
  2. The bacteria will become resistant to antibiotics
  3. The healthcare team may not receive the result
  4. The result becomes automatically invalid
32) A technologist discovers that a culture plate was incubated at the wrong temperature overnight. What is the most appropriate action?
  1. Ignore it if colonies are visible
  2. Inform the supervisor and document the issue
  3. Re-incubate the plate at the correct temperature
  4. Report all results as preliminary
33) A technologist notices that a final report was released with an incorrect organism name. What should the technologist do?
  1. Correct the report and notify appropriate personnel
  2. Delete the incorrect report from the system
  3. Do nothing because the report was already released
  4. Request a new patient sample and notify the patient
34) A CSF Gram stain shows gram-negative diplococci. What should the laboratory do?
  1. Report as normal flora
  2. Report the result as a critical value
  3. Request a new sample
  4. Wait until full identification before reporting
35) A patient has the following lipid panel results.
Test Result
Total cholesterol 160 mg/dL
LDL 100 mg/dL
HDL 40 mg/dL
Calculate the total cholesterol-to-HDL cholesterol ratio.
  1. 0.25
  2. 4
  3. 50
  4. 150
36) A patient's cholesterol results are as follows.
Test Result
Triglycerides 200 mg/dL
Cholesterol 300 mg/dL
HDL 70 mg/dL
Use the Friedewald equation to calculate the patient's LDL.
  1. 190 mg/dL
  2. 200 mg/dL
  3. 210 mg/dL
  4. 220 mg/dL
37) Which of these chemistry results fails the CLIA quality requirements for proficiency testing?
  1. Blood gas pH: 1 above the target value
  2. Sodium: 2 mmol/L below the target value
  3. Total calcium: 0.5 mg/dL below the target value
  4. Total cholesterol: 4% above the target value
38) The Beer–Lambert Law states that absorbance =
  1. concentration × (molar absorptivity / path length)
  2. concentration × (path length / molar absorptivity)
  3. molar absorptivity × (concentration / path length)
  4. molar absorptivity × concentration × path length
39) A patient's serum protein results are as follows.
Test Result
Total protein 6.6 g/dL
Albumin 3.3 g/dL
Alpha₁ 0.2 g/dL
Alpha₂ 0.7 g/dL
Beta 1.1 g/dL
Gamma 1.3 g/dL
Calculate the patient's A/G ratio.
  1. 0.9
  2. 1.0
  3. 1.1
  4. 1.2
40) The hematology analyzer gives a critical hemoglobin result. What is the best action?
  1. Notify the appropriate clinical team
  2. Request a new specimen
  3. Rerun the sample to verify the result
  4. Wait for the other tests to be complete
41) A technician forgot to perform quality control on a hematology analyzer. What is the main concern?
  1. Patient results may be inaccurate
  2. The analyzer's warranty may be voided
  3. The laboratory will lose its accreditation
  4. The technician may have accidentally been exposed to a biological agent
42) What does it mean if a lab value is critical?
  1. The patient may have a life-threatening condition
  2. The test is malfunctioning and needs to be calibrated
  3. The value is needed to calculate other lab results
  4. The value must be reported to the doctor within 24 hours
43) How should proficiency testing samples be tested?
  1. Before patient samples
  2. By a reference laboratory
  3. By a senior technologist
  4. The same as regular specimens
44) A laboratory compares its staining quality with results from other laboratories. This is an example of:
  1. competency assessment
  2. internal quality control
  3. method validation
  4. proficiency testing
45) Which of these situations would cause random errors?
  1. Reading liquid volumes from different angles
  2. Using a balance that was not zeroed before use
  3. Using a scale that always reads 10 g too low
  4. Using a thermometer that always reads 2°C too high
46) What is the term for when readings become consistently lower or higher over time?
  1. Calibration
  2. Drift
  3. Offset error
  4. Unpredictability
47) While counting the number of colonies on an agar plate, a lab technician accidentally counts the same colony twice. What type of error is this?
  1. Environmental
  2. Instrumental
  3. Observational
  4. Theoretical
48) Which of these is a pre-analytical error?
  1. Calculating test results incorrectly
  2. Interpreting results incorrectly
  3. Mislabeling a blood specimen
  4. Reporting a result on the wrong patient
49) Which of these is a zero error?
  1. A blood sample missing a label
  2. A lab technician forgetting a zero when performing a calculation
  3. A thermometer registering 102°C in boiling water instead of 100°C
  4. Scales that give a reading of 1.1 g when nothing is placed on them
50) Which of these is a post-analytical error?
  1. A hemolyzed specimen
  2. An empty collection tube
  3. Incorrect information on the test request
  4. Results reported in the wrong units
51) Which of these is an example of a systematic error?
  1. A specimen tube is mislabeled
  2. Splashover contaminates a gel card well
  3. The incubator temperature is consistently too hot
  4. The technician makes a typo when entering a result into the LIS
52) Random error mainly affects:
  1. accuracy
  2. external validity
  3. internal validity
  4. precision
53) A technologist notices that anti-D control reactions have gradually weakened over several days. What kind of error is this?
  1. Clerical
  2. Post-analytical
  3. Random
  4. Systematic
54) What is the term for a solution with a precisely known concentration?
  1. Benchmark
  2. Control
  3. Model
  4. Standard
55) Checking and adjusting a measuring instrument against a known standard is called:
  1. calibration
  2. estimation
  3. graduation
  4. mensuration
56) What are quality indicators?
  1. Measures used to monitor performance
  2. Physical strips that change color to reveal a chemical state
  3. Reference samples used to verify the accuracy of tests
  4. Tools used to adjust the accuracy of equipment
57) Which of the following is a quality assurance indicator?
  1. C-reactive protein test
  2. Fume hoods
  3. Laboratory turnaround time
  4. Thorough records of exposure incidents
58) A technologist performs corrective maintenance on an automated analyzer. What should the technologist do before resuming patient testing?
  1. Decontaminate the interior with bleach
  2. Re-run the last five patient samples
  3. Run proficiency testing
  4. Run quality control
59) Why are controls tested with patient specimens?
  1. To calculate the concentration of the patient's analyte
  2. To calibrate the instrument
  3. To ensure the testing process is working properly
  4. To establish the patient’s reference range
60) The result of a blind duplicate should:
  1. closely agree with the original result
  2. fall below the detection limit of the instrument
  3. fall within the reference interval for the test
  4. match the result of the positive control
61) A substance with a known value that is used to ensure the validity and accuracy of test results is called a:
  1. control
  2. reagent
  3. reference
  4. sample
62) What is the difference between a standard and a control?
  1. Standards are prepared by the laboratory, while controls are provided by the manufacturer
  2. Standards are used for calibration, while controls check that the test is working correctly
  3. Standards have unknown concentrations, while controls have known concentrations
  4. Standards measure test precision, while controls measure test accuracy
63) Where would you find the instructions for running quality controls for a specific assay?
  1. Laboratory quality manual
  2. Safety data sheet
  3. Safety manual
  4. Standard operating procedure
64) Below is a blood bank control chart for anti-D reagent QC:
Day Reaction strength
1 4+
2 4+
3 4+
4 2+
5 2+
6 2+
What is the name for this pattern?
  1. Dispersion
  2. Random error
  3. Shift
  4. Trend
65) What kind of chart is a graphical presentation of run-to-run or day-to-day quality control values?
  1. Eadie-Hofstee
  2. Hanes-Woolf
  3. Levey-Jennings
  4. Lineweaver-Burk
66) What is a commercial control?
  1. A calibration standard made in the laboratory
  2. A control prepared from pooled patient specimens
  3. A control purchased from a manufacturer
  4. A control used solely for marketing demonstrations
67) What is the purpose of a blind duplicate?
  1. To determine the detection limit
  2. To establish a reference range
  3. To evaluate consistency between results
  4. To identify matrix interferences
68) Which federal agency provides calibration services for thermometers?
  1. American Association for Clinical Chemistry (AACC)
  2. American Clinical Laboratory Association (ACLA)
  3. Department of Energy (DOE)
  4. National Institute of Standards and Technology (NIST)
69) The systematic monitoring and evaluation of the entire laboratory testing process is an aspect of:
  1. corporate compliance
  2. critical thinking
  3. quality assurance
  4. risk management
70) Which term means the extent to which measurements agree with the true value?
  1. Accuracy
  2. Precision
  3. Reliability
  4. Reproducibility
71) If the same test is repeated several times and always gives the same result, the test is:
  1. accurate
  2. reliable
  3. valid
  4. variable
72) When is data considered reliable?
  1. When the instruments provide precise measurements
  2. When it is close to the true value
  3. When it is statistically significant
  4. When repeated measurements yield similar results
73) Precise values:
  1. are caused by random errors
  2. are close to the true value
  3. are the same as the true value
  4. fall within a narrow range
74) How is diagnostic sensitivity calculated?
  1. False negatives / (false negatives + true positives)
  2. False positives / (false positives + true negatives)
  3. True negatives / (true negatives + false positives)
  4. True positives / (true positives + false negatives)
75) What is the purpose of calibrating equipment?
  1. To ensure accurate and reliable measurements
  2. To ensure employees understand how to use equipment
  3. To prevent laboratory-acquired infections
  4. To protect workers from harm
76) What should a laboratory do if it receives proficiency testing samples from another laboratory for testing?
  1. Forward the samples to an outside laboratory
  2. Notify the inspecting authority
  3. Test the samples immediately
  4. Test the samples the same way you test patient specimens
77) Which of these best meets the definition of proficiency testing?
  1. Inspections to prevent work-related accidents
  2. Testing for illegal drugs in a biological sample
  3. Tests to ensure workers have the skills and knowledge to perform their jobs
  4. The testing of unknown samples sent to the laboratory
78) A proficiency testing sample arrives during a busy shift. What should the technologist do with the specimen?
  1. Give it to the most experienced technologist
  2. Process it the same way as patient specimens
  3. Test it first, before other specimens
  4. Test it last, after all other work is complete
79) What is the purpose of external quality assessment?
  1. To calibrate new laboratory instruments
  2. To evaluate a laboratory's performance
  3. To replace the need for daily controls
  4. To track the expiration dates of reagents
80) What is the purpose of proficiency testing?
  1. To evaluate a laboratory's testing accuracy
  2. To monitor the rate of equipment malfunctions
  3. To track the inventory levels of laboratory reagents
  4. To train new employees on laboratory techniques
81) The quality control chart of an instrument shows ten consecutive data points on one side of the mean. What should you do?
  1. Accept the run
  2. Reject the run and follow the method's troubleshooting procedures
  3. Repeat the control sera on the next run
  4. Report the results with a disclaimer that they may be erroneous
82) In a control run, five consecutive measurements fall between the mean and +1 SD. What should the lab technician do?
  1. Accept the control run
  2. Accept the control run, but make a note of the incident in the log file
  3. Reject the control run and take corrective action
  4. Rerun the control run
83) In a run of twenty control samples, a single control value falls between 2 and 3 standard deviations from the mean. The other control readings are within the normal range, and no source of error can be identified. What is the most appropriate action to take?
  1. Inform the clinician that results are likely to be incorrect
  2. Prepare a new reagent
  3. Replace the equipment
  4. Report patient results
84) A laboratory accidentally releases a report with an incorrect blood group result. What should the laboratory do?
  1. Amend the report and notify the clinical staff
  2. Delete the original report and issue a new report
  3. Request a new sample and issue a new report
  4. Retest the original sample and issue a new report
85) During pretransfusion testing, the patient's ABO blood group does not match the historical record. What is the most appropriate action?
  1. Change the historical record to match the new result
  2. Report the current ABO result as the patient's blood group
  3. Report the historical ABO result as the patient's blood group
  4. Resolve the discrepancy
86) A laboratory switches from using a highly toxic chemical to a less toxic alternative. Which safety principle has the laboratory applied?
  1. Administrative controls
  2. Elimination
  3. Engineering controls
  4. Substitution
87) An AHG reagent fails daily QC, so the technologist opens a new vial, which passes QC. However, the technologist only documents the passed QC, not the failed QC. Why is this a problem?
  1. All results will be legally invalid
  2. The new vial is now contaminated
  3. The test is now inaccurate
  4. There is no record of the failure
88) Below is a blood bank control chart for anti-D reagent QC:
Day Reaction strength
1 4+
2 4+
3 2+
4 4+
5 4+
6 4+
What does this pattern most likely suggest?
  1. Random error
  2. Shift
  3. Systematic error
  4. Trend
89) Which action could constitute battery?
  1. Disclosing a patient's health information without consent
  2. Holding down a patient to draw their blood against their will
  3. Refusing to draw blood from a patient who arrives late to the appointment
  4. Spreading malicious rumours about a colleague
90) A patient forgets to collect one urine sample during a 24-hour urine collection. What should usually happen?
  1. Add extra urine
  2. Estimate the volume of the missed sample
  3. Restart the 24-hour collection
  4. Submit the container and write “one sample missed” on the requisition
91) A courier delivers a chain of custody sample to a technician at a laboratory. Who needs to sign and date the chain of custody form?
  1. Only the courier
  2. Only the laboratory technician
  3. The courier and the laboratory technician
  4. The laboratory manager and the laboratory technician
92) Which of the following is NOT required on a chain of custody form?
  1. The name of the patient's doctor
  2. The name of the person who collected the specimen
  3. The time the specimen was collected
  4. The type of specimen collected
93) What should the laboratory do if a chain-of-custody form is missing?
  1. Ask the courier to complete a new form
  2. Fill out a new form yourself
  3. Reject the specimen
  4. Test the specimen, but add a comment
94) Which problem with a chain-of-custody form would automatically result in rejection of the specimen?
  1. The courier's name is missing
  2. The specimen ID does not match the ID on the form
  3. The specimen temperature box is unticked
  4. There is a digit error in the patient's ID number
95) Which of these tests requires chain of custody documentation?
  1. Complete blood count
  2. Fetal fibronectin
  3. Free light chains test
  4. Legal paternity test
96) A urine specimen for a drug screen arrives in the laboratory. However, the seal of the specimen bottle is broken. What should you do?
  1. Ask the courier to reseal the bottle
  2. Reject the specimen
  3. Test the specimen, but add a comment
  4. Transfer the urine to a new, sterile container
97) A blood sample is unlabeled, but a nurse states that she knows which patient it belongs to. What is the best action?
  1. Label it using the nurse’s information
  2. Let the nurse label it herself
  3. Reject it and request a new sample
  4. Test it, but with a comment about the missing label
98) A blood sample arrives at the laboratory, but the name on the tube does not match the requisition. What should the laboratory do?
  1. Accept the sample, but write a note in the LIS
  2. Ask the phlebotomist to correct the label
  3. Correct the label
  4. Request a new sample
99) Which of these is a reason for rejecting a CBC specimen?
  1. The patient was fasting
  2. The specimen is at room temperature
  3. The specimen is clotted
  4. The specimen was collected in a lavender tube
100) A glucose specimen was left unprocessed at room temperature and discovered 3 hours later. What is the best action?
  1. Add a glycolysis inhibitor and run the sample
  2. Centrifuge the specimen and run the sample
  3. Request a new sample
  4. Shake the tube and run the sample
101) A laboratory fails to notify the doctor of a critical result. What type of error is this?
  1. Analytical
  2. Post-analytical
  3. Pre-analytical
  4. Quality control failure
102) Which of these is required when documenting a critical result communication?
  1. The name of the laboratory director
  2. The patient's date of birth
  3. The patient’s room number
  4. The person notified
103) A technologist notices that a critical result was entered into the LIS but not phoned, even though the SOP requires phone notification. What should the technologist do?
  1. Delete the result
  2. Document that the result is in the LIS
  3. Nothing, because the result is already in the computer
  4. Phone the result and document the notification
104) A lab technologist amends a report after an error is found. What should the technologist document?
  1. Only the corrected result
  2. The correction, department, laboratory telephone number, and person who made the change
  3. The correction, patient's ID number, and technologist's initials
  4. The correction, reason for amendment, date, time, and person who made the change
105) A laboratory audit reveals frequent delays in critical result reporting. Which intervention would most effectively solve this problem?
  1. Eliminating verbal communication
  2. Implementing escalation protocols
  3. Increasing documentation requirements
  4. Retraining staff on sample collection techniques
106) A laboratory issues blood for transfusion, but then discovers a mistake in the blood typing results. What should the laboratory do first?
  1. Contact the clinical area
  2. Delete the issue record and create a new record
  3. Repeat testing on the original sample
  4. Request a new sample
107) A technologist accidentally enters a susceptibility result under the wrong patient record. What is the most important concern?
  1. The laboratory’s turnaround time will increase
  2. The microorganisms could die before susceptibility testing occurs
  3. The report may be delayed
  4. The wrong patient may receive inappropriate treatment
108) You phone a frozen section diagnosis to the operating room. What is the most important action before ending the call?
  1. Ask the receiver to read back the result
  2. Confirm the patient's preferred pronouns
  3. Document the call in the laboratory information system
  4. Politely thank the receiver
109) According to OSHA, which vaccine must be offered to employees at risk of exposure to bloodborne pathogens?
  1. HIV
  2. Hepatitis A
  3. Hepatitis B
  4. MMR
110) What is the most effective type of hazard control in the ISO 45001 Hierarchy of Controls?
  1. Administrative controls
  2. Elimination
  3. Engineering controls
  4. Substitution
111) Which danger must employers inform workers about under OSHA’s Hazard Communication Standard?
  1. Bloodborne pathogens
  2. Dangerous chemicals
  3. General safety hazards
  4. Miscommunication
112) Which GHS pictogram is not mandatory under OSHA?
  1. Environment
  2. Exclamation mark
  3. Flame over circle
  4. Gas cylinder
113) In OSHA, a(n) __________ person is defined as "one who is capable of identifying existing and predictable hazards in the surroundings or working conditions which are unsanitary, hazardous, or dangerous to employees, and who has the authorization to take prompt corrective measures to eliminate them."
  1. authorized
  2. capable
  3. competent
  4. qualified
114) What type of hazard are OSHA action levels for?
  1. Chemicals and noise
  2. Ergonomic hazards
  3. Psychosocial hazards
  4. Slips, trips, and falls
115) What is the least effective type of hazard control in the ISO 45001 Hierarchy of Controls?
  1. Administrative controls
  2. Engineering controls
  3. Personal protective equipment
  4. Substitution
116) What is an exempt employee?
  1. An employee who does not have to pay income taxes
  2. An employee who is exempt from overtime rules
  3. An employee working on a temporary or seasonal basis
  4. An employee working remotely
117) Title I, Employment, is part of which civil rights law prohibiting discrimination?
  1. Americans with Disabilities Act (ADA)
  2. Civil Rights Act
  3. Equal Employment Opportunity Act (EEOA)
  4. Occupational Safety and Health Administration (OSHA)
118) Which federal law, passed in 1972, amended Title VII of the Civil Rights Act of 1964 to strengthen protections against employment discrimination?
  1. Employment Non-Discrimination Act (ENDA)
  2. Equal Employment Opportunity Act (EEOA)
  3. Equal Pay Act
  4. Rehabilitation Act
119) Which act regulates whether employees are exempt or nonexempt?
  1. Civil Rights Acts
  2. Equal Pay Act (EPA)
  3. Fair Labor Standards Act (FLSA)
  4. Occupational Safety and Health Act (OSHA)
120) How many weeks of unpaid leave does the Family and Medical Leave Act (FMLA) allow employees within a 12-month period without jeopardizing their job?
  1. 12 weeks
  2. 20 weeks
  3. 24 weeks
  4. 36 weeks
121) What legal term describes a person who is not of sound mind and is considered legally incapable of responsibility for their actions?
  1. Corpus juris
  2. Habeas corpus
  3. Non compos mentis
  4. Res ipsa loquitur
122) Which legal term means the degree of care a prudent, reasonable healthcare provider would exercise in a given situation?
  1. Customary care
  2. Optimal care
  3. Scope of practice
  4. Standard of care
123) What could a physician be held liable for if he fails to inform the patient of the risks and benefits of a procedure?
  1. Assault
  2. Battery
  3. Fraud
  4. Negligence
124) What federal agency regulates the manufacturing of prescription medications?
  1. American Dental Association (ADA)
  2. Drug Enforcement Administration (DEA)
  3. Food and Drug Administration (FDA)
  4. Occupational Safety and Health Administration (OSHA)
125) What federal agency administers and enforces the Occupational Safety and Health Act?
  1. Environmental Protection Agency (EPA)
  2. National Institute for Occupational Safety and Health (NIOSH)
  3. Occupational Safety and Health Administration (OSHA)
  4. United States Public Health Service (USPHS)
126) Which federal agency regulates the transport of hazardous materials?
  1. Centers for Disease Control and Prevention (CDC)
  2. Department of Transportation (DOT)
  3. Food and Drug Administration (FDA)
  4. National Highway Traffic Safety Administration (NHTSA)
127) Which federal agency regulates clinical laboratories through the CLIA program?
  1. Center for Global Health (CGH)
  2. Centers for Medicare and Medicaid Services (CMS)
  3. Health Resources and Services Administration (HRSA)
  4. Office of Public Health Science (OPHS)
128) Which agency enforces the HIPAA Privacy and Security Rules?
  1. The Attorney General
  2. The Office for Civil Rights
  3. The Surgeon General
  4. The US Senate
129) Which agency provides procedures and reagents for rapidly detecting biothreats and emerging infectious diseases?
  1. Centers for Disease Control and Prevention (CDC)
  2. Centers for Medicare & Medicaid Services (CMS)
  3. Environmental Protection Agency (EPA)
  4. Occupational Safety and Health Administration (OSHA)
130) Which federal agency regulates in vitro diagnostic tests?
  1. Federal Laboratory Consortium (FLC)
  2. Food and Drug Administration (FDA)
  3. National Institutes of Health (NIH)
  4. Occupational Safety and Health Administration (OSHA)
131) Which federal agency investigates complaints of job discrimination?
  1. Equal Employment Opportunity Commission (EEOC)
  2. Fair Labor Association (FLA)
  3. Food and Drug Administration (FDA)
  4. Occupational Safety and Health Administration (OSHA)
132) Which federal agency sets the standards and guidelines for Hospital, Medical, and Infectious Waste Incinerators (HMIWI)?
  1. Centers for Disease Control and Prevention (CDC)
  2. Environmental Protection Agency (EPA)
  3. Food and Drug Administration (FDA)
  4. Occupational Safety and Health Administration (OSHA)
133) Which federal agency requires employers to provide safety training to their employees?
  1. National Institute for Occupational Safety and Health (NIOSH)
  2. Occupational Safety and Health Administration (OSHA)
  3. United States Department of the Interior (USDI)
  4. United States Office of Personnel Management (USOPM)
134) Which federal agency enforces workplace safety laws?
  1. Food and Drug Administration (FDA)
  2. National Institutes of Health (NIH)
  3. Occupational Safety and Health Administration (OSHA)
  4. Office of Public Health Science (OPHS)
135) Which federal agency regulates commercially marketed in vitro diagnostic tests?
  1. American Clinical Laboratory Association (ACLA)
  2. Clinical Laboratory Improvement Amendments (CLIA)
  3. Food and Drug Administration (FDA)
  4. Occupational Safety and Health Administration (OSHA)
136) Which federal agency requires employers to provide workers with PPE?
  1. Centers for Disease Control and Prevention (CDC)
  2. National Institute for Occupational Safety and Health (NIOSH)
  3. National Institutes of Health (NIH)
  4. Occupational Safety and Health Administration (OSHA)
137) What federal agency categorizes clinical laboratory tests by their complexity?
  1. Centers for Disease Control and Prevention (CDC)
  2. Clinical Laboratory Improvement Amendments (CLIA)
  3. Food and Drug Administration (FDA)
  4. Occupational Safety and Health Administration (OSHA)
138) What federal agency regulates general-purpose surface disinfectants?
  1. American Medical Association (AMA)
  2. Drug Enforcement Administration (DEA)
  3. Environmental Protection Agency (EPA)
  4. Food and Drug Administration (FDA)
139) Which federal agency enforces federal employment discrimination laws, such as the Civil Rights Act and the Americans with Disabilities Act (ADA)?
  1. Employee Benefits Security Administration (EBSA)
  2. Employment and Training Administration (ETA)
  3. Equal Employment Opportunity Commission (EEOC)
  4. Occupational Safety and Health Administration (OSHA)
140) Which federal agency regulates laboratory testing performed on humans?
  1. American Medical Association (AMA)
  2. Centers for Disease Control and Prevention (CDC)
  3. Centers for Medicare & Medicaid Services (CMS)
  4. Food and Drug Administration (FDA)
141) Which law sets the federal minimum wage?
  1. Civil Rights Act
  2. Equal Pay Act
  3. Fair Labor Standards Act (FLSA)
  4. National Labor Relations Act (NLRA)
142) Which federal law prohibits employment discrimination based on race, color, religion, sex, and national origin?
  1. Americans with Disabilities Act
  2. Civil Rights Act
  3. Fair Labor Standards Act (FLSA)
  4. National Labor Relations Act (NLRA)
143) Which law requires laboratories to document the competency of their personnel?
  1. Clinical Laboratory Improvement Amendments (CLIA)
  2. Fair Labor Standards Act (FLSA)
  3. National Labor Relations Act (NLRA)
  4. Occupational Safety and Health Act (OSHA)
144) Which law requires healthcare providers to ensure effective communication with deaf patients, such as providing a qualified interpreter?
  1. Americans with Disabilities Act (ADA)
  2. Family and Medical Leave Act (FMLA)
  3. Health Care Right of Conscience Act (HCRCA)
  4. Health Insurance Portability and Accountability Act (HIPAA)
145) Which act outlines the lawful use and disclosure of protected health information?
  1. Digital Accountability and Transparency Act (DATA)
  2. Freedom of Information Act (FOIA)
  3. Health Insurance Portability and Accountability Act (HIPAA)
  4. Privacy Act
146) Which act gives workers the right to know about hazards in the workplace?
  1. Health Care Consent Act (HCCA)
  2. Occupational Safety and Health Act
  3. Privacy Act
  4. SAFETY Act
147) Which law requires safeguards when sending health information by email?
  1. CAN-SPAM Act
  2. Health Insurance Portability and Accountability Act (HIPAA)
  3. Occupational Safety and Health Act (OSHA)
  4. Privacy Act
148) Which OSHA standard did the Needlestick Safety and Prevention Act amend?
  1. Bloodborne Pathogens Standard
  2. Confined Space Entry Standard
  3. Hazard Communication Standard
  4. Personal Protective Equipment Standard
149) Which act prevents employers from retaliating against workers for complaining about unsafe working conditions?
  1. Fair Labor Standards Act (FLSA)
  2. Federal Employees' Compensation Act (FECA)
  3. Occupational Safety and Health Act (OSHA)
  4. Uniformed Services Employment and Reemployment Rights Act (USERRA)
150) Under the American National Standards Institute (ANSI) standards, which safety sign indicates a potential hazard that may result in minor or moderate injury?
  1. Caution
  2. Danger
  3. Notice
  4. Warning
151) The Globally Harmonized System of Classification and Labeling of Chemicals has two signal words for hazardous chemicals: "Danger" and:
  1. Attention
  2. Caution
  3. Notice
  4. Warning
152) How often does CLIA require routine inspections of non-waived laboratories?
  1. Every three months
  2. Every six months
  3. Every year
  4. Every two years
153) According to CLIA, who is responsible for the day-to-day supervision of the laboratory operation and its personnel?
  1. General supervisor
  2. Laboratory director
  3. Technical supervisor
  4. Testing personnel
154) How often does CLIA require calibration verification of test systems?
  1. Every three months
  2. Every six months
  3. Every year
  4. Every two years
155) When are laboratories exempt from proficiency testing?
  1. When they are CLIA certified
  2. When they have a Certificate of Compliance
  3. When they have six or fewer employees
  4. When they only perform waived tests
156) Under CLIA regulations, which of these is a high-complexity test?
  1. Complete blood count
  2. Drugs of abuse testing
  3. Electrolyte profile
  4. Examination of a blood smear
157) According to CLIA, who is responsible for specimen processing, test performance, and reporting test results?
  1. Laboratory director
  2. Technical consultant
  3. Technical supervisor
  4. Testing personnel
158) Which CLIA certificate allows laboratories to conduct moderate or high complexity tests before the laboratory is inspected and a final certificate is issued?
  1. Accreditation
  2. Compliance
  3. Registration
  4. Waiver
159) Under CLIA regulations, how many categories of lab test complexity are there?
  1. 3
  2. 5
  3. 6
  4. 8
160) What does a Certificate of Waiver allow a facility to do?
  1. Conduct tests without patient consent
  2. Perform simple, low-risk laboratory tests
  3. Store controlled substances without a DEA license
  4. Use RoHS-exempt equipment
161) According to CLIA, who is responsible for the technical and scientific oversight of the laboratory?
  1. Laboratory technician
  2. Medical equipment repairer
  3. Technical consultant
  4. Test system manufacturer
162) CLIA refers to moderate and high complexity tests collectively as _____________ tests.
  1. cleared
  2. licensed
  3. nonwaived
  4. point-of-care
163) Which of these tests is most likely to be CLIA-waived?
  1. Complete blood count
  2. Immunoassay
  3. Peripheral smear
  4. Urine hCG test
164) According to CLIA, who is responsible for the overall operation and administration of the laboratory?
  1. Clinical consultant
  2. Laboratory director
  3. Technical consultant
  4. Technical supervisor
165) When should laboratories use alternative assessment?
  1. For highly complex tests
  2. For tests that have critical values
  3. When a traditional proficiency test is not available
  4. When the laboratory is not yet CLIA-certified
166) According to CLIA, how many areas of competency must be included in a laboratory competency assessment program?
  1. 6
  2. 7
  3. 8
  4. 9
167) Which CLIA category of test complexity does dipstick urinalysis fall under?
  1. High complexity
  2. Moderate complexity
  3. Provider performed microscopy
  4. Waived
168) What is mixed with a weak acid or a weak base to create a buffer?
  1. A salt solution
  2. A strong acid or strong base
  3. Acetic acid
  4. The conjugate salt
169) How many times more acidic is pH 5 than pH 6?
  1. 1
  2. 2
  3. 10
  4. 100
170) Which term describes a substance that minimizes changes in pH?
  1. Anhydride
  2. Buffer
  3. Compound
  4. Solvent
171) What is the term for a solution with a pH value of less than 7?
  1. Acidic
  2. Alkaline
  3. Basic
  4. Neutral
172) Which ion does pH measure?
  1. Carbon
  2. Hydrogen
  3. Nitrogen
  4. Oxygen
173) Which pH is neutral?
  1. 0
  2. 1
  3. 3
  4. 7
174) For data distributed normally, what percentage of values fall within one standard deviation of the mean?
  1. 50%
  2. 68%
  3. 83%
  4. 95%
175) In a normal distribution, what percentage of cases are above the mean?
  1. 50%
  2. 68%
  3. 75%
  4. 100%
176) The following data were calculated on a series of 100 determinations of a control.
Mean 5.8
Median 6.0
Standard deviation 0.25
If control limits are set at ±2 standard deviations, what are the allowable limits for the control?
  1. 5.30–6.30
  2. 5.35–6.25
  3. 5.50–5.90
  4. 5.60–5.90
177) For data distributed normally, what percentage of values fall within two standard deviations of the mean?
  1. 50%
  2. 68%
  3. 83%
  4. 95%
178) If a data set has a mean of 7.2 and a standard deviation of 1.2, what range of values falls within two standard deviations of the mean?
  1. 4.8–9.6
  2. 6.0–8.4
  3. 7.2–9.6
  4. 8.6–10.2
179) For data distributed normally, what percentage of values fall within three standard deviations of the mean?
  1. 68%
  2. 83%
  3. 95%
  4. 99.7%
180) Assuming values are normally distributed, what range should encompass 95% of observed values if a control has a mean of 5 and a standard deviation of 1?
  1. 1–9
  2. 2–8
  3. 3–7
  4. 4–6
181) Which formula calculates the coefficient of variation?
  1. (Mean / standard deviation) × 100
  2. (Standard deviation / mean) × 100
  3. (Standard deviation / variance) × 100
  4. (Variance / mean) × 100
182) Automated white blood cell counts are performed on 29 blood samples. The mean value is 8.0 cells/nL, and the standard deviation is 1.2 cells/nL. What is the coefficient of variation?
  1. 15%
  2. 30%
  3. 45%
  4. 60%
183) If the mean is 20 and two standard deviations is 8, what is the coefficient of variation?
  1. 0.2%
  2. 4%
  3. 20%
  4. 40%
184) Ten patients have the following bilirubin results: 3.1, 3.2, 3.8, 4.1, 4.7, 5.1, 5.9, 6.0, 6.2, 6.9. Calculate the mean of these results.
  1. 4.5
  2. 4.7
  3. 4.9
  4. 5.1
185) What is the term for the middle value in a sorted data set?
  1. Average
  2. Coefficient of variation
  3. Mean
  4. Median
186) What is the term for the value that appears most often in a set of data values?
  1. Average
  2. Coefficient of variation
  3. Mean
  4. Mode
187) Where should bulk quantities of acetone be kept?
  1. Biological safety cabinet
  2. Flammable storage cabinet
  3. Freezer
  4. Refrigerator
188) How is diethyl ether stored?
  1. In airtight containers
  2. In the light
  3. In the refrigerator
  4. Underwater
189) Where should stock ethanol solutions be stored?
  1. Flammable safety cabinet
  2. Freezer
  3. Fume hood
  4. Refrigerator
190) Under OSHA's Hazard Communication Standard, which of the following information is mandatory on hazardous chemical labels?
  1. Environmental pictogram
  2. Expiration date
  3. Personal protective equipment (PPE) pictogram
  4. Phone number of the manufacturer or importer
191) If a chemical has a hazard that warrants a “Danger” signal word and another hazard that warrants the “Warning” signal word, then what signal word should appear on the label?
  1. "Fatal"
  2. Both "Warning" and "Danger"
  3. Only the “Danger” signal word
  4. Only the “Warning” signal word
192) A small acetone spill occurs on the bench near a lit Bunsen burner. What should be the first action?
  1. Clean up the spill
  2. Move everyone to fresh air
  3. Turn off the flame
  4. Wash eyes with water
193) Which of these substances would be the best solution to clean up a large blood spill?
  1. 1:10 dilution of bleach
  2. 70% isopropyl alcohol
  3. Antibacterial soap and water
  4. Povidone-iodine
194) Which concentration of bleach solution is used to decontaminate benchtops?
  1. 10%
  2. 20%
  3. 30%
  4. 50%
195) What does cold sterilization use to sterilize medical instruments?
  1. Chemicals
  2. Cold air
  3. Ice
  4. Liquid nitrogen
196) What is the first step when cleaning up a blood spill?
  1. Pour disinfectant onto the spill
  2. Put on PPE
  3. Record the incident in the incident book
  4. Wash your hands
197) Which of these ethyl alcohol dilutions is the most effective disinfectant?
  1. 10% ethyl alcohol
  2. 40% ethyl alcohol
  3. 70% ethyl alcohol
  4. 100% ethyl alcohol
198) Which chemical is used to decontaminate equipment exposed to prions?
  1. Alcohol
  2. Formaldehyde
  3. Hydrogen peroxide
  4. Sodium hydroxide
199) Which cleaning product releases a toxic gas when used to clean urine spills?
  1. Chlorine bleach
  2. Hydrogen peroxide
  3. Isopropyl alcohol
  4. Povidone-iodine
200) Which chemical is best for neutralizing weak acid spills?
  1. Acetic acid
  2. Boric acid
  3. Sodium bicarbonate
  4. Sodium hydroxide
201) Mercury spills are most dangerous when the spill occurs:
  1. in a fume hood
  2. in a poorly ventilated area
  3. near sources of ignition
  4. near the flammable storage cabinet
202) Which of these is used for cleaning minor liquid radioactive spills?
  1. Bleach
  2. Lead sheet
  3. Paper towels
  4. Soda ash
203) What pressure and temperature are commonly used for steam sterilization in an autoclave?
  1. 12 psi at 115°C
  2. 12 psi at 120°C
  3. 15 psi at 112°C
  4. 15 psi at 121°C
204) Which type of glass is autoclavable?
  1. Borosilicate
  2. Float
  3. Lead
  4. Soda-lime
205) Which process kills all microorganisms, including spores?
  1. Cleaning
  2. Disinfection
  3. Sanitization
  4. Sterilization
206) Why should a dry-heat sterilizer remain closed throughout the sterilization cycle?
  1. To avoid damaging the oven
  2. To ensure complete sterilization
  3. To prevent moisture from entering the chamber
  4. To prevent oxygen from entering the chamber
207) Which of these is a Standard Precaution?
  1. Fire extinguisher
  2. Hand hygiene
  3. Keeping chemical containers closed
  4. Radiation shielding
208) What is the meaning of a 4 in the yellow quadrant of an NFPA hazard diamond?
  1. The substance is highly radioactive
  2. The substance is extremely contagious
  3. The substance readily burns at normal temperatures
  4. The substance readily explodes
209) Which color on the NFPA hazard diamond signals the degree of hazards to health?
  1. Blue
  2. Green
  3. White
  4. Yellow
210) What is the meaning of a 1 in the red quadrant on the NFPA hazard diamond?
  1. The substance does not burn
  2. The substance only ignites after considerable preheating
  3. The substance ignites if moderately heated
  4. The substance can be ignited at normal temperatures
211) On the NFPA hazard diamond below, what do the letters SA mean?
  1. Can shock or cause acid damage
  2. Safety assessed
  3. Simple asphyxiant
  4. Substance abuse
212) What does a number 2 in the blue quadrant of an NFPA hazard diamond indicate?
  1. Continued exposure to the substance could cause temporary incapacitation or injury
  2. The substance can be ignited at normal temperatures
  3. The substance is unreactive with water
  4. The substance reacts with water in an unusual or dangerous way
213) In the NFPA hazard diamond, which quadrant is for special hazards?
  1. Blue
  2. Red
  3. White
  4. Yellow
214) Which prefix means 10⁻⁶?
  1. Centi-
  2. Kilo-
  3. Micro-
  4. Nano-
215) What does the prefix kilo- mean?
  1. 10
  2. 100
  3. 1,000
  4. 10,000
216) What does the prefix nano- mean?
  1. 1 hundredth
  2. 1 thousandth
  3. 1 millionth
  4. 1 billionth
217) What does the symbol μ represent in SI units?
  1. 10⁹
  2. 10⁻³
  3. 10⁻⁶
  4. 10⁻⁹
218) What are cubic decimeters more commonly known as?
  1. Grams
  2. Liters
  3. Meters cubed
  4. Moles
219) Which prefix means 0.001?
  1. Micro-
  2. Centi-
  3. Pico-
  4. Milli-
220) What is 0.05 mL in microliters?
  1. 0.0005 μL
  2. 0.005 μL
  3. 5 μL
  4. 50 μL
221) What is the following graph shape?
  1. Normal distribution
  2. Polynomial function
  3. Quadratic chart
  4. Reciprocal graph
222) Which federal agency regulates the disposal of hazardous waste produced by laboratories?
  1. Centers for Disease Control and Prevention (CDC)
  2. Centers for Medicare & Medicaid Services (CMS)
  3. Environmental Protection Agency (EPA)
  4. Food and Drug Administration (FDA)
223) Which act regulates hazardous waste disposal?
  1. Clean Water Act (CWA)
  2. National Environmental Policy Act (NEPA)
  3. Pollution Prevention Act (PPA)
  4. Resource Conservation and Recovery Act (RCRA)
224) Which of these is regulated waste?
  1. Empty medicine bottles
  2. Gauze packaging
  3. Sterile dressings
  4. Used blood tubes
225) Where should you discard used needles?
  1. Biohazard sharps container
  2. Glass disposal bucket
  3. Glass recycling bin
  4. Regular garbage
226) What is an upper flammability limit?
  1. The amount of fuel that will burn before the fuel burns itself out
  2. The highest concentration of a vapor that can catch fire
  3. The highest temperature at which a liquid can be stored safely
  4. The highest temperature that a chemical can reach when ignited
227) Under OSHA regulations, a flammable liquid has a flash point at or below which temperature?
  1. 37.8°C
  2. 67.3°C
  3. 93°C
  4. 100°C
228) Refer to the image below. Which of these fires can this fire extinguisher extinguish?
  1. Cardboard boxes
  2. Electrical equipment
  3. Methanol
  4. Oil bath
229) A fire extinguisher has the following symbol. This fire extinguisher:
  1. can be used on fires involving flammable liquids or gases
  2. requires annual top-ups of type B fuel
  3. should be stored in a separate room from flammable chemicals
  4. uses kerosene to extinguish fires
230) What are the three components of the fire triangle?
  1. Oxygen, heat, and fuel
  2. Oxygen, ignition, and fuel
  3. Rescue, Alarm, Escape
  4. Rescue, Alarm, Extinguish
231) What does the acronym RACE mean in the context of fire procedures?
  1. Ready, Aim, Check, Extinguish
  2. Recover, Act, Control, Extinguish/Evacuate
  3. Rescue, Alarm, Confine, Extinguish/Evacuate
  4. Run, Activate, Coordinate, Extinguish/Evacuate
232) A fire extinguisher has the following symbol: This fire extinguisher:
  1. can extinguish electrical fires
  2. cannot be used on electrical equipment
  3. carries a risk of electric shock
  4. requires charging before use
233) Which of these chemicals is flammable?
  1. Acetone
  2. Carbon tetrachloride
  3. Chloroform
  4. Saline
234) Where should paraformaldehyde solutions be prepared?
  1. In a biological safety cabinet
  2. In a chemical fume hood
  3. In the sink
  4. Under a laminar flow clean bench
235) A 500 mL bottle of 35% formaldehyde falls on the floor and breaks. What should be your first action?
  1. Dilute the spill with water
  2. Evacuate the area
  3. Put on PPE
  4. Record the incident in the incident log
236) At low concentrations, what does hydrogen sulfide smell like?
  1. Burnt sugar
  2. Rotten eggs
  3. Rotting meat
  4. Strong cheese
237) When mixing acid and water:
  1. add the acid quickly to the water
  2. add the acid slowly to the water
  3. add the water quickly to the acid
  4. add the water slowly to the acid
238) Where should you handle chemicals that produce harmful vapors?
  1. Autoclave
  2. Biological safety cabinet
  3. Fume hood
  4. Sealed room
239) What should be your first action in the event of an acid burn?
  1. Cover the burn with gauze moistened in sterile saline
  2. Flush the burn with water
  3. Go to the emergency department
  4. Neutralize the acid with sodium bicarbonate
240) What is the minimum amount of time the eyes should be flushed with water in the event of a chemical splash?
  1. 1 minute
  2. 5 minutes
  3. 10 minutes
  4. 15 minutes
241) A lab technologist has spilled concentrated hydrochloric acid onto his clothing and skin, affecting a large portion of his body. After he has removed his clothing, what is the next thing he should do?
  1. Apply a burn ointment to his skin
  2. Pour baking soda onto his skin
  3. Seek emergency medical assistance
  4. Use the emergency safety shower
242) Which type of hazard do universal precautions apply to?
  1. Bloodborne pathogens
  2. Dangerous chemicals
  3. Environmental hazards
  4. Radiation
243) Which biosafety level does a nonpathogenic strain of E. coli require?
  1. BSL-1
  2. BSL-2
  3. BSL-3
  4. BSL-4
244) Which of these is an OPIM (other potentially infectious material)?
  1. Blood
  2. Semen
  3. Urine
  4. Vomit
245) The responsibility for identifying employees at risk of exposure to blood or OPIM lies with the:
  1. employer
  2. government
  3. patient
  4. union
246) What is the pictogram for a pressurized gas?
  1. A flame
  2. A flame over a circle
  3. A gas cylinder
  4. An exploding test tube
247) Which of these is found on safety data sheets?
  1. Expiry date
  2. Health hazards
  3. Price
  4. Production date
248) How many sections are in a standard GHS safety data sheet?
  1. 8
  2. 10
  3. 12
  4. 16
249) Which centrifuge is used to spin capillary tubes?
  1. Angle head
  2. Cytospin
  3. Microhematocrit
  4. Refrigerated
250) Which centrifuge concentrates cells from a fluid specimen onto a microscope slide for morphological examination?
  1. Angle head
  2. Cytocentrifuge
  3. Microhematocrit
  4. Refrigerated
251) What should you do before turning on a centrifuge?
  1. Ensure the centrifuge is balanced
  2. Open the cover
  3. Place the centrifuge on a slanted surface
  4. Uncover all liquid specimen tubes
252) What does RPM indicate on a centrifuge?
  1. How fast the centrifuge spins
  2. How long the centrifuge spins for
  3. How many tubes the centrifuge can hold
  4. The maximum weight the rotor can safely handle
253) Which device measures the RPM of a centrifuge?
  1. Ohmmeter
  2. Rheostat
  3. Tachometer
  4. Voltmeter
254) What is the most common cause of excessive centrifuge vibration?
  1. Electrical interference
  2. Unbalanced tubes
  3. Uneven bench surfaces
  4. Variable voltage
255) What is the term for water from which charged particles have been removed?
  1. Chlorinated water
  2. De-ionized water
  3. Distilled water
  4. Flat water
256) What is the term for a solution in which no more solute can be dissolved?
  1. Concentrated
  2. Diluted
  3. Saturated
  4. Soluted
257) Which microscope is used most in clinical laboratories?
  1. Acoustic
  2. Electron
  3. Light
  4. Scanning probe
258) Which part of a microscope controls the brightness of the light bulb?
  1. Diaphragm
  2. Numerical aperture
  3. Rheostat
  4. Stage clip
259) Which part of a microscope controls how much light reaches the specimen?
  1. Diaphragm
  2. Numerical aperture
  3. Objective lens
  4. Rheostat
260) Which objective lenses do compound light microscopes usually have?
  1. 2×, 10×, 50×, and 100×
  2. 2×, 20×, 50×, and 80×
  3. 4×, 10×, 40×, and 100×
  4. 5×, 20×, 40×, and 80×
261) Which microscope is best for examining an unstained, wet preparation of cells before dehydration?
  1. Brightfield
  2. Electron
  3. Fluorescence
  4. Phase contrast
262) Which part of a microscope focuses light onto the specimen?
  1. Condenser
  2. Diaphragm
  3. Objective lens
  4. Ocular lens
263) Which magnification is typically used to examine the morphology of bacteria?
  1. 10x
  2. 100x
  3. 400x
  4. 1000x
264) You notice dirt in the field of view while using a microscope. The dirt moves when you rotate the eyepiece. What action should you take?
  1. Clean the eyepiece
  2. Clean the objective
  3. Open the iris
  4. Tighten the tension adjustment
265) A pipette gives a reading of 4.9 mL, but the true value of the volume of liquid inside the pipette is 5.0 mL. What is the percent error?
  1. 2%
  2. 4%
  3. 6%
  4. 8%
266) Which item of glassware is designed to deliver specific volumes?
  1. Beaker
  2. Erlenmeyer flask
  3. Volumetric flask
  4. Volumetric pipette
267) Which pipette is the most accurate?
  1. Graduated
  2. Mohr
  3. Pasteur
  4. Volumetric
268) What does "10 mL TD 25 SEC" on a pipette mean?
  1. The pipette can be filled with 10 mL in 25 seconds
  2. The pipette can deliver 10 mL in its primary tube and 25 mL in its secondary tube
  3. The pipette is calibrated to deliver 10 mL after a 25-second drain time
  4. You should wait 25 seconds between filling and dispensing the pipette
269) A to-deliver pipettor has an accuracy of +/- 0.8%. When using the pipette to deliver a volume of 100 µL, what range would you expect the volume of liquid to fall within?
  1. 99.05–100.95 µL
  2. 99.2–100.8 µL
  3. 99.55–100.45 µL
  4. 99.85–100.15 µL
270) What do the double rings mean on this pipette?
  1. The last drop must be blown out
  2. The pipette can hold 2 mL
  3. The pipette will deliver the measured amount of liquid only at 20°C
  4. The volume is measured at this point
271) Which pipette has a bulged-out portion in the middle?
  1. Mohr
  2. Pasteur
  3. Serological
  4. Volumetric
272) At which level should a volumetric pipette be viewed for an accurate reading?
  1. At an angle
  2. At eye level
  3. Just above eye level
  4. Just below eye level
273) Which glassware is used to measure 24-hour urine volumes?
  1. Beaker
  2. Erlenmeyer flask
  3. Graduated cylinder
  4. Graduated pipette
274) Which type of pipette is used to measure and dispense various volumes using calibration marks (graduations) along the entire length of the tube?
  1. Disposable
  2. Pasteur
  3. Serological
  4. Volumetric
275) What does a biological safety cabinet protect against?
  1. Infectious aerosols
  2. Thermal burn injuries
  3. UV radiation
  4. Volatile chemical vapors
276) How often should biological safety cabinets be certified?
  1. Once a year
  2. Every 2 years
  3. Every 3 years
  4. Every 4 years
277) What type of filter in biological safety cabinets removes airborne particles such as bacteria and viruses?
  1. Electrostatic
  2. Fiberglass
  3. HEPA
  4. UV light
278) Which grade of chemical is used in analytical laboratory work that requires high-purity chemicals?
  1. AR
  2. CP
  3. LR
  4. USP
279) Which hazard category do formalin, xylene, and other reagents fall into?
  1. Biological
  2. Chemical
  3. Magnetic
  4. Radiation
280) Which situation should be documented as a laboratory incident?
  1. A culture plate is moved to the incubator
  2. A specimen arrives leaking
  3. A technician takes a scheduled 15-minute break
  4. The LIS autoverifies results
281) Which of these actions reduces the risk of repetitive strain injury?
  1. Repeating the same movement many times
  2. Resting your arms on a hard surface
  3. Sitting in the same position for a long period of time
  4. Taking frequent breaks
282) What is the name of the process of assessing the severity and likelihood of workplace hazards?
  1. Health needs evaluation
  2. Myers-Briggs type indicator
  3. Risk assessment
  4. Workplace danger evaluation
283) What does the mnemonic PASS help to remember?
  1. The key safety principles of working in a laboratory
  2. The steps for first aid
  3. The steps to operate a fire extinguisher
  4. The warning signs of a stroke
284) What is the term for a document that identifies hazards and the ways to minimize their impact?
  1. Accident and incident recording form
  2. First aid assessment form
  3. Inspection report
  4. Risk assessment
285) What is the universal solvent?
  1. 0.85% sodium chloride
  2. 10% ethanol
  3. 8.5% sodium chloride
  4. Water
286) Which of these describes preventive maintenance?
  1. Identifying unexpected failures
  2. Repairing equipment after it fails
  3. Servicing equipment before it fails
  4. Turning off machines when they are not in use
287) Which of these chemical grades has the highest purity?
  1. Commercial
  2. Laboratory
  3. Reagent
  4. Technical
288) Which equipment measures small masses with high precision?
  1. Analytical balance
  2. Desiccator
  3. PH meter
  4. Single pan balance
289) What is the function of a buffer?
  1. To accentuate changes in pH
  2. To make a solution more acidic
  3. To make a solution more basic
  4. To minimize changes in pH
290) Triple-beam balances give readings in what unit of measurement?
  1. Grams
  2. Kilograms
  3. Liters
  4. Milliliters
291) Which pH buffer solution is typically used first when calibrating a pH meter?
  1. pH 1
  2. pH 4
  3. pH 7
  4. pH 10
292) What substance is used to calibrate refractometers?
  1. Buffer solution
  2. Distilled water
  3. Healthy urine
  4. Hydrochloric acid
293) Which water purification method involves boiling water into steam, and then letting the steam cool?
  1. Deionization
  2. Distillation
  3. Filtration
  4. Reverse osmosis
294) How is relative centrifugal force (RCF) converted from revolutions per minute (RPM)?
  1. RCF = RPM × 1.144 × 10⁵ × r²
  2. RCF = RPM × 1.144 × 10⁻⁵ × r²
  3. RCF = RPM² × 1.118 × 10⁵ × r
  4. RCF = RPM² × 1.118 × 10⁻⁵ × r
295) Which of these best defines 'turnaround time'?
  1. The duration of a blood cell count
  2. The time from specimen receipt to result reporting
  3. The time required for sample preparation
  4. The time taken for instrument maintenance
296) Solid crystals of potassium oxalate are added to distilled water in a container. Which term describes the potassium oxalate?
  1. Reagent
  2. Solute
  3. Solution
  4. Solvent
297) What is a delta check?
  1. A comparison of a laboratory's results to those from an outside facility
  2. A comparison of current test results to previous results
  3. An inspection to ensure all patients are wearing wristbands
  4. The verification of a patient's identity using at least three identifiers
298) Which of these actions would increase a laboratory's efficiency?
  1. Increasing the time it takes to perform key tasks
  2. Keeping frequently used equipment within easy reach
  3. Using an inconsistent labeling system
  4. Using paper files instead of digital files
299) What is the term for a solution that contains less solute than the maximum amount of solute that can be dissolved?
  1. Diluted
  2. Saturated
  3. Supersaturated
  4. Unsaturated
300) A hypertonic solution:
  1. causes cells to grow
  2. contains a higher concentration of solutes than another solution
  3. contains a lower concentration of solutes than another solution
  4. contains the same concentration of solutes as another solution

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