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ASCP PBT Practice Questions

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About the ASCP PBT exam

The ASCP Phlebotomy Technician (PBT) certification exam assesses knowledge of phlebotomy. The exam is set by the American Society for Clinical Pathology (ASCP).

About these practice questions

These practice questions will help prepare you for the ASCP PBT exam.

This page contains 400 practice questions divided into the six sections of the exam: 1. Circulatory system, 2. Specimen collection, 3. Specimen handling, transport, and processing, 4. Waived and point-of-care-testing, 5. Non-blood specimens, and 6. Laboratory operations.

All questions have been carefully designed to mimic the questions on the real exam, to help you prepare and get a passing grade.

Sections

  1. Circulatory system
  2. Specimen collection
  3. Specimen handling, transport, and processing
  4. Waived and point-of-care-testing
  5. Non-blood specimens
  6. Laboratory operations

Section 1: Circulatory system

1.1) Which vein is the median cubital?
  1. a
  2. b
  3. c
  4. d
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1.2) What is the term for an immature red blood cell?
  1. Erythroblast
  2. Monoblast
  3. Segmented
  4. Thrombocyte
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1.3) What is macrocytosis?
  1. A blood cancer
  2. A high concentration of white blood cells
  3. A platelet deficiency
  4. Abnormally large red blood cells
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1.4) Which blood vessel carries blood from the heart to the lungs?
  1. Aorta
  2. Pulmonary artery
  3. Pulmonary vein
  4. Superior vena cava
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1.5) What is the name of the process by which platelets adhere to one another and form a hemostatic plug?
  1. Activation
  2. Aggregation
  3. Attachment
  4. Hemostasis
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1.6) What term means the destruction of red blood cells?
  1. Erythropoiesis
  2. Hemolysis
  3. Hemorrhage
  4. Hemostasis
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1.7) Plasma makes up around _____ of the total blood.
  1. 55%
  2. 65%
  3. 75%
  4. 95%
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1.8) Bilirubin is a waste product produced by the breakdown of which cells?
  1. Mast cells
  2. Platelets
  3. Red blood cells
  4. White blood cells
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1.9) What is the major hematopoietic site in adults?
  1. Bone marrow
  2. Liver
  3. Lymph nodes
  4. Spleen
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1.10) Which blood cell is typically the first to appear in the response to an infection?
  1. Basophil
  2. Eosinophil
  3. Erythrocyte
  4. Neutrophil
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1.11) Which blood cells defend the body against disease?
  1. Erythrocytes
  2. Hematocytes
  3. Leukocytes
  4. Thrombocytes
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1.12) Natural killer cells are what type of white blood cell?
  1. Basophil
  2. Eosinophil
  3. Lymphocyte
  4. Monocyte
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1.13) Blood returning from the lungs enters which part of the heart?
  1. Left atrium
  2. Left ventricle
  3. Right atrium
  4. Right ventricle
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1.14) The heart is contained inside a sac called the:
  1. endocardium
  2. fluid sac
  3. myocardium
  4. pericardium
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1.15) What is the largest vein in the body?
  1. Aorta
  2. Hepatic portal vein
  3. Inferior vena cava
  4. Pulmonary vein
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1.16) In the image below, which part of the heart is the left ventricle?
  1. a
  2. b
  3. c
  4. d
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1.17) What are the smallest blood vessels in the body?
  1. Arteries
  2. Capillaries
  3. Lymph vessels
  4. Veins
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1.18) Which is the largest artery in the body?
  1. Aorta
  2. Iliac artery
  3. Pulmonary artery
  4. Superior vena cava
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1.19) Systolic blood pressure is the blood pressure when which part of the heart contracts?
  1. Arteries
  2. Atria
  3. Valves
  4. Ventricles
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1.20) What is diastole?
  1. A heart rate below 60 beats per second
  2. An irregular heart rate
  3. When the heart muscle contracts
  4. When the heart muscle relaxes
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1.21) What is this blood cell?
  1. Macrophage
  2. Neutrophil
  3. Platelet
  4. Red blood cell
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1.22) Phagocytes:
  1. carry oxygen around the body
  2. form clots to stop bleeding
  3. ingest pathogens
  4. release antibodies against pathogens
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1.23) Which component of blood carries oxygen?
  1. Granulocytes
  2. Hemoglobin
  3. Platelets
  4. White blood cells
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1.24) White blood cells are also known as:
  1. erythrocytes
  2. leukocytes
  3. osteocytes
  4. thrombocytes
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1.25) What is the blood cell below?
  1. Macrophage
  2. Neutrophil
  3. Platelet
  4. Red blood cell
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1.26) Red blood cells are also known as:
  1. eosinophils
  2. erythrocytes
  3. monocytes
  4. thrombocytes
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1.27) When blood is anticoagulated and centrifuged, the resulting straw-colored liquid layer is:
  1. heparin
  2. plasma
  3. serum
  4. whole blood
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1.28) The liquid that separates out when blood coagulates is:
  1. fibrinogen
  2. lymph
  3. plasma
  4. serum
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1.29) Which of these is found in plasma but not in serum?
  1. Albumin
  2. Antibodies
  3. Fibrinogen
  4. White blood cells
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1.30) When an anticoagulated blood sample is centrifuged, it separates into three layers. What is the middle layer called?
  1. Buffy coat
  2. Erythrocyte layer
  3. Leukocyte layer
  4. Plasma
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1.31) Capillary blood composition most closely resembles:
  1. arterial blood
  2. lymph fluid
  3. tissue fluid
  4. venous blood
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1.32) A deficiency of red blood cells is called:
  1. anemia
  2. erythremia
  3. hemophilia
  4. leukemia
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1.33) Which vein is located on the thumb side of the arm?
  1. Cephalic
  2. External iliac
  3. Femoral
  4. Posterior tibial
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Section 2: Specimen collection

2.1) Which of these specimens has the highest priority?
  1. Fasting
  2. Post-op
  3. Stat
  4. Urgent
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2.2) You have three specimens to collect: routine, preop, and fasting. What is the correct order of priority for collecting these specimens?
  1. Fasting, Preop, Routine
  2. Fasting, Routine, Preop
  3. Preop, Fasting, Routine
  4. Routine, Fasting, Preop
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2.3) Which of these specimens has the lowest priority?
  1. Postop
  2. Preop
  3. Routine
  4. Timed
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2.4) A patient is extremely anxious about having her blood drawn. She tells you that she is afraid of needles. You do not have much experience drawing blood. What should you do?
  1. Ask an experienced phlebotomist to perform the draw for you
  2. Explain to her that you will use a small needle that barely hurts
  3. Tell her that it's not a big deal and that she shouldn't be afraid
  4. Use an ice pack to numb the site before drawing the specimen
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2.5) There are two patients in a room and one of the patients has a latex allergy. A phlebotomist needs to collect a specimen from the other patient. How should the phlebotomist proceed?
  1. Ask the allergic patient to wear a mask
  2. Do not take anything that contains latex into the room
  3. Proceed as usual
  4. Pull the curtain between beds and proceed as usual
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2.6) When drawing blood from a frightened child, which of these is the right thing to do?
  1. Explain the procedure to the child in simple terms
  2. Say nothing to the child and just perform the procedure
  3. Tell the child that the needle won't hurt
  4. Tell the child to be brave
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2.7) If a patient refuses to have blood drawn:
  1. ask the patient for a urine sample instead
  2. notify the patient's nurse or physician
  3. restrain the patient and draw the blood
  4. try to convince the patient to be cooperative
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2.8) A priest is with the patient when you arrive to collect a stat specimen. What should you do?
  1. Draw blood from a different patient and then check back
  2. Excuse yourself and ask for permission to draw the specimen
  3. Go back to the lab and draw the specimen on the next sweep
  4. Wait outside the room for a few minutes
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2.9) A phlebotomist needs to draw an urgent blood sample from a patient who is asleep. What should the phlebotomist do?
  1. Cancel the test
  2. Come back later when the patient is awake
  3. Draw the sample while the patient is asleep
  4. Wake up the patient
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2.10) When drawing a patient's blood, which of these steps should come first?
  1. Apply the tourniquet
  2. Clean the site
  3. Identify the patient
  4. Insert the needle
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2.11) A phlebotomist is asked to collect a blood specimen from an inpatient but the patient is not wearing an ID band. What is the best thing to do?
  1. Ask a nurse for help identifying the patient
  2. Identify the patient by asking them to confirm their name
  3. Identify the patient by the name card on the door
  4. Refuse to draw the specimen and cancel the request
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2.12) You have to draw blood from an elderly patient. You think his name is John Smith but you are not sure. Which of these is the best question to confirm the patient's name?
  1. "Are you John Smith?"
  2. "Can you confirm that you are John Smith please?"
  3. "I'm a phlebotomist and I'm here to draw your blood. First, though, I have to confirm your name. Can you confirm that your name is John Smith?"
  4. "What is your name?"
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2.13) When drawing blood from an obese patient, which location should be the first place you look for a vein?
  1. The antecubital fossa
  2. The back of the knee
  3. The feet
  4. The neck
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2.14) Which of these actions can help a phlebotomist find a difficult vein?
  1. Ask the patient to hold their arm up in the air
  2. Ask the patient to make a fist
  3. Cool the area with an ice pack
  4. Tell the patient to drink nothing and come back in a few hours
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2.15) There are several short lengths of IV-type tubing protruding from a patient's chest. This is most likely a:
  1. CVC
  2. PICC
  3. arterial line
  4. implanted port
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2.16) A patient has an IV in his left wrist and a cast on his right arm. Which site should be used to obtain a blood sample?
  1. A vein in the left hand
  2. An earlobe
  3. The left median cubital vein
  4. The right median cubital vein
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2.17) A patient has a mastectomy on the right side. What special precaution is needed when drawing blood from this patient?
  1. Check the patient's white blood cell count first
  2. Do not draw blood from the right arm
  3. Make sure the patient is lying down
  4. Make sure the patient is sitting
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2.18) Which vein should be tried first when choosing a draw site?
  1. Basilic
  2. Cephalic
  3. Median antibrachial
  4. Median cubital
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2.19) A patient has an arteriovenous (AV) fistula in his left arm and an IV in his right arm. From where should you draw a blood sample?
  1. Above the AV fistula
  2. Above the IV
  3. Below the AV fistula
  4. Below the IV
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2.20) Tourniquets should be:
  1. applied very tightly to the arm
  2. left on the arm for at least three minutes
  3. tight enough to slow arterial flow
  4. tight enough to slow venous flow
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2.21) In venipuncture, why must you wait for the alcohol on the puncture site to dry before inserting the needle into the vein?
  1. To ensure all pathogens are killed
  2. To ensure the needle doesn't slip
  3. To prevent alcohol from entering the patient's bloodstream
  4. To prevent hemolysis of the specimen
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2.22) Why must a phlebotomist clean the patient's skin before performing a venipuncture?
  1. To allow blood flow to the area
  2. To cause the veins to be more prominent
  3. To decrease circulation in the arteries
  4. To remove microorganisms from the puncture site
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2.23) What is the purpose of a tourniquet in venipuncture?
  1. To decrease the oxygen level
  2. To make the veins more prominent
  3. To monitor blood pressure
  4. To prevent hematoma
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2.24) What is the most common antiseptic used in venipuncture?
  1. 70% isopropyl alcohol
  2. Betadine
  3. EMLA
  4. Iodine
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2.25) After applying a tourniquet, small red spots appear on the patient's arm. These spots are a sign that:
  1. the patient has diabetes
  2. the patient is taking an anticoagulant
  3. the site may bleed excessively
  4. the tourniquet is too tight
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2.26) For venipuncture, the tourniquet should be applied ________________ the needle insertion point.
  1. 1-2 inches above
  2. 1-2 inches below
  3. 3-4 inches above
  4. 3-4 inches below
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2.27) When a blood pressure cuff is used for a venipuncture instead of a tourniquet, the pressure must be:
  1. between the patient's diastolic and systolic blood pressure
  2. equal to the patient's systolic blood pressure
  3. just above the patient's systolic blood pressure
  4. just below the patient's diastolic blood pressure
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2.28) What can happen if a phlebotomist releases the tourniquet after removing the needle from the arm?
  1. Angina
  2. Bleeding
  3. Cyanosis
  4. Edema
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2.29) What might happen if an angle of 8 degrees is used when performing venipuncture?
  1. A deep hematoma may form
  2. The needle may enter above the vein
  3. The needle may go completely through the vein
  4. The needle may go through the posterior wall of the vein
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2.30) What can happen if a phlebotomist uses an angle of 35 degrees when performing venipuncture?
  1. Nothing will happen as this is the correct angle
  2. The needle may go completely through the vein
  3. The needle may miss the vein completely
  4. The needle will enter above the vein
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2.31) Which of these statements about venipuncture is true?
  1. After collecting the blood, remove the needle first, then the tourniquet
  2. Do not leave the tourniquet on for more than 1 minute
  3. Enter the vein at a 50-degree angle
  4. The bevel of the needle should be pointing down when the needle enters the vein
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2.32) What should you do if a patient faints during a venipuncture?
  1. Continue the procedure until all blood is collected
  2. Leave the needle in the vein and call the physician
  3. Remove the needle and attend to the patient
  4. Yell loudly at the patient to keep him conscious
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2.33) In venipuncture, which of these actions may cause a hematoma?
  1. Applying pressure to the puncture site after removing the needle
  2. Asking the patient to form a fist so the veins are more prominent
  3. Inserting the needle through the vein and puncturing the opposite wall
  4. Removing the tourniquet before the needle is removed
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2.34) During phlebotomy, in which direction should the bevel be facing when the needle enters the arm?
  1. Downwards
  2. To the left
  3. To the right
  4. Upwards
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2.35) A phlebotomist has made two unsuccessful attempts while trying to collect a blood specimen from a patient. What should the phlebotomist do next?
  1. Ask another phlebotomist to collect it
  2. Ask the patient's nurse to do the draw
  3. Collect it by arterial puncture
  4. Try to draw it one more time
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2.36) Which of these is a sign that an artery has been punctured instead of a vein during phlebotomy?
  1. The blood is dark blue
  2. The blood spurts into the tube
  3. The patient feels intense pain
  4. The patient has trouble breathing
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2.37) Which of these would be most likely to allow reflux to occur during venipuncture?
  1. Filling the stopper end of the tube first
  2. Lateral redirection of the needle
  3. Releasing the tourniquet on blood flow
  4. Using the wrong order of draw
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2.38) Which of these situations can result in hemoconcentration during venipuncture?
  1. Drawing a large tube with a small needle
  2. Leaving the tourniquet on for over 1 minute
  3. Mixing the specimen too vigorously
  4. Partially filling a normal-draw tube
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2.39) A phlebotomist is performing a venipuncture. The needle is in place but no blood is entering the tube. What is the first thing the phlebotomist should do?
  1. Change to pediatric tubes
  2. Discontinue the draw and cancel the requisition
  3. Switch to a winged blood collection set
  4. Try adjusting the needle slightly
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2.40) When a phlebotomist inserts a needle into the patient's arm, bright red blood spurts into the vacutainer. What does this indicate?
  1. A high hematocrit
  2. A low blood pH
  3. An arterial puncture
  4. Carbon monoxide poisoning
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2.41) Which of these actions helps to prevent hemoconcentration during venipuncture?
  1. Asking the patient to make and release a fist several times
  2. Asking the patient to release her fist upon blood flow
  3. Keeping the tourniquet on for longer than a minute
  4. Massaging the site before inserting the needle
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2.42) When performing venipuncture with a syringe, what could happen if you pull the plunger up too quickly when collecting the specimen?
  1. A needle stick injury
  2. Adulteration of the specimen
  3. Bacterial contamination
  4. Hemolysis of the specimen
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2.43) During venipuncture, what is the longest time a tourniquet should remain on the patient's arm?
  1. 1 minute
  2. 3 minutes
  3. 5 minutes
  4. 7 minutes
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2.44) Which of these tests is affected most if collected from a crying infant?
  1. Bilirubin
  2. Cholesterol
  3. Lead level
  4. White blood cell count
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2.45) When drawing blood, when should the phlebotomist remove the tourniquet?
  1. After removing the needle from the arm
  2. As soon as a suitable vein has been found
  3. Ten minutes after applying the tourniquet
  4. While the last tube to be drawn is filling
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2.46) Which complication could occur if a phlebotomist punctures a baby's calcaneus?
  1. Achilles tendon rupture
  2. Hyperglycaemia
  3. Osteomyelitis
  4. Petechiae
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2.47) A phlebotomist is collecting blood from the vein of a 14-month-old baby when the blood stops flowing. Which of these actions should the phlebotomist take to try to continue the draw?
  1. Give the baby something sweet to eat
  2. Release the tourniquet
  3. Try a new tube
  4. Try to collect blood from the baby's heel instead
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2.48) Repeated phlebotomy procedures in the same area can cause:
  1. petechiae
  2. scarring
  3. thrombosis
  4. varicose veins
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2.49) Which vacutainer tube is for collecting a blood sample for a Factor IX assay?
  1. Gray
  2. Lavender
  3. Light blue
  4. SST
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2.50) Which of these vacutainer tubes does NOT contain an anticoagulant?
  1. Green
  2. Lavender
  3. Light blue
  4. Red
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2.51) A physician requests a vitamin A test and a complete blood count. Which vacutainer tubes should the phlebotomist use to collect the blood samples for these tests?
  1. Gold and lavender
  2. Gray and gold
  3. Gray and lavender
  4. Gray and light blue
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2.52) Sodium heparin should be avoided for which of these tests?
  1. Electrolyte panel
  2. Hematocrit
  3. Lithium levels
  4. pH
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2.53) A phlebotomist has to collect a purple top tube, green top tube, and light blue top tube from the same patient. In which order should the phlebotomist fill the tubes?
  1. Green, light blue, purple
  2. Green, purple, light blue
  3. Light blue, green, purple
  4. Purple, green, light blue
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2.54) Westergren tubes are used for which test?
  1. Erythrocyte sedimentation rate
  2. Hematocrit
  3. Microhematocrit
  4. Reticulocyte count
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2.55) Which tube would be drawn for an antinuclear antibody test (ANA)?
  1. Gray
  2. Green
  3. Red
  4. SST
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2.56) What are cross-match tubes used for?
  1. Blood compatibility testing
  2. Coagulation studies
  3. Glucose tests
  4. Trace element studies
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2.57) EDTA prevents clotting by binding to:
  1. anti-hemophilia factor
  2. calcium ions
  3. fibrinogen
  4. prothrombin
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2.58) Blue-top coagulation tubes require a citrate-to-blood ratio of:
  1. 1:3
  2. 1:6
  3. 1:9
  4. 1:12
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2.59) Which anticoagulant is used for blood gas tests?
  1. EDTA
  2. Heparin
  3. Potassium oxalate
  4. Trisodium citrate
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2.60) What anticoagulant is in a green top vacutainer?
  1. Citrate
  2. EDTA
  3. Fluoride
  4. Heparin
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2.61) What special action does a phlebotomist need to take before drawing a blue top tube with a butterfly needle?
  1. Draw a discard tube
  2. Get a bucket of ice ready
  3. Label the tube
  4. Make sure the patient is lying down
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2.62) A phlebotomist needs to collect blood samples from a patient for a blood glucose test and a hemoglobin test. What color tubes should the phlebotomist use?
  1. Gray and green
  2. Gray and lavender
  3. Gray and red
  4. Red and green
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2.63) Gray-top vacutainers contain which anticoagulant?
  1. Heparin
  2. Potassium oxalate
  3. SPS
  4. Sodium polyanethole sulfonate
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2.64) What additive do light blue top tubes contain?
  1. EDTA
  2. Heparin
  3. Sodium citrate
  4. Sodium fluoride
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2.65) What are lavender top tubes used for?
  1. Blood cultures
  2. Erythrocyte sedimentation rates
  3. Glucose determinations
  4. Hematology tests
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2.66) Which anticoagulant tube is needed to collect a sample for an HbA1c test?
  1. EDTA
  2. Heparin
  3. Sodium citrate
  4. Sodium fluoride
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2.67) What is the glycolytic inhibitor in gray-top tubes?
  1. Heparin
  2. Silicon
  3. Sodium citrate
  4. Sodium fluoride
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2.68) Which vacutainer tube is used for trace metal analysis?
  1. Dark blue
  2. Green
  3. Orange
  4. Red
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2.69) What color Vacutainer tube is used for erythrocyte sedimentation rates?
  1. Black
  2. Green
  3. Red
  4. Yellow
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2.70) What vacutainer tube is used for coagulation studies?
  1. Black
  2. Gray
  3. Green
  4. Light blue
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2.71) What vacutainer tube is used for reticulocyte counts?
  1. Green
  2. Lavender
  3. Light blue
  4. Red
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2.72) Lavender-top collection tubes are used for:
  1. glucose determinations
  2. hematology tests
  3. immunology tests
  4. molecular diagnostic tests
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2.73) A phlebotomist needs to draw blood into a gray-top tube, a gold-top tube and a lavender-top tube. What is the correct order to fill the tubes?
  1. Gold, gray, lavender
  2. Gold, lavender, gray
  3. Gray, gold, lavender
  4. Gray, lavender, gold
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2.74) Which color vacutainer tube is used for glucose testing?
  1. Gray
  2. Green
  3. Light blue
  4. Red
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2.75) What color tube is used for stat chemistry tests?
  1. Green
  2. Light blue
  3. Red
  4. Yellow
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2.76) Light blue top tubes are used for what tests?
  1. Chemistry
  2. Coagulation
  3. Hematology
  4. Serology
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2.77) For which tube is the blood-to-additive ratio most critical?
  1. Green top
  2. Lavender top
  3. Light blue top
  4. Red top
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2.78) Which of these tubes contains an anticoagulant that works by binding calcium?
  1. Green top
  2. Light blue top
  3. PST
  4. Red top
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2.79) Serum specimens are collected in which vacutainer tube?
  1. Green
  2. Light blue
  3. PST
  4. SST
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2.80) A phlebotomist has to draw a patient's blood into a blood culture bottle and four tubes: red, green, light blue, and purple. Which should the phlebotomist fill first?
  1. Blood culture bottle
  2. Light blue top tube
  3. Purple top tube
  4. Red top tube
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2.81) A phlebotomist is performing venipuncture on a difficult vein using a butterfly. The phlebotomist has to collect an SST and a light blue-topped tube. How should the phlebotomist proceed?
  1. Collect the SST first then the light blue top tube
  2. Collect the light blue top tube first then the SST
  3. Draw a clear tube first, then collect the light blue top tube, then collect the SST
  4. Draw half of the SST first, then collect the light blue top tube, then finish the SST
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2.82) Underfilling which tube will most likely result in a hemolyzed specimen?
  1. EDTA tube
  2. Gray top
  3. Light-blue top
  4. SST
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2.83) Why are infant bilirubin specimens obtained in amber-colored tubes?
  1. To flag it as a capillary specimen
  2. To identify it as a bilirubin specimen
  3. To protect the specimen from light
  4. To reduce the risk of hemolysis
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2.84) A phlebotomist has finished drawing blood into a citrate tube for PT testing. However, the blood in the tube is below the minimum line. The phlebotomist should:
  1. fill another citrate tube partly full and mix the two tubes
  2. pour a little blood from another tube into the citrate tube
  3. repeat the procedure with a new citrate tube
  4. submit the tube for testing as usual
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2.85) Blood for a white blood cell count would generally be collected in which color vacutainer?
  1. Black
  2. Blue
  3. Gray
  4. Lavender
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2.86) Which of these tests is collected in a light blue top tube?
  1. Differential
  2. Hematocrit
  3. Platelet count
  4. Prothrombin time
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2.87) Which of these tubes contains EDTA?
  1. Gray
  2. Lavender
  3. Light blue
  4. Light green
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2.88) What color is the top of a plasma separator tube?
  1. Green
  2. Light blue
  3. Purple
  4. Yellow
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2.89) You have collected blood in a tube containing an additive. What should you do next?
  1. Empty the tube into the sink
  2. Invert the tube several times
  3. Leave the tube in the fridge for 2-3 hours
  4. Shake the tube vigorously
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2.90) EDTA prevents blood clots by binding:
  1. calcium
  2. chloride
  3. plasma
  4. red blood cells
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2.91) Lavender top tubes contain which additive?
  1. EDTA
  2. Lithium heparin
  3. Sodium fluoride
  4. Sodium heparin
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2.92) Yellow-topped tubes contain which additive?
  1. Acid citrate dextrose
  2. Sodium benzoate
  3. Sodium nitrite
  4. Sulfur dioxide
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2.93) Which of these additives provides a physical barrier to prevent glycolysis?
  1. EDTA
  2. Silica
  3. Sodium fluoride
  4. Thixotropic gel
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2.94) Which vacutainer tube has a light yellow top?
  1. PST
  2. SPS
  3. SST
  4. Sodium heparin
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2.95) When a test requires plasma, the blood sample must be collected in a tube containing a(n):
  1. anticoagulant
  2. antiglycolytic agent
  3. chelating agent
  4. clot activator
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2.96) Venipuncture needles are color-coded according to their:
  1. expiration date
  2. gauge
  3. length
  4. manufacturer
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2.97) Which of these would be the best choice of equipment for drawing blood from a small hand vein?
  1. A 21-gauge needle and ETS holder
  2. A 21-gauge needle and syringe
  3. A 23-gauge butterfly and ETS holder
  4. A 27-gauge butterfly and syringe
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2.98) Which tool is needed to collect blood by syringe?
  1. Multisample needle
  2. Transfer device
  3. Tube holder
  4. Winged infusion set
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2.99) Which of these needles has the largest diameter?
  1. 18-gauge
  2. 19-gauge
  3. 20-gauge
  4. 21-gauge
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2.100) A winged infusion set is also called a:
  1. Milliner's needle
  2. butterfly needle
  3. straight needle
  4. universal needle
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2.101) 24-gauge needles are used for:
  1. diabetics
  2. infants
  3. patients with AIDS
  4. stroke victims
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2.102) What is the standard needle size for collecting a blood sample from an adult's forearm vein?
  1. 15-gauge
  2. 18-gauge
  3. 21-gauge
  4. 24-gauge
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2.103) Which part of a needle enters the skin first?
  1. Bevel
  2. Hub
  3. Lumen
  4. Shaft
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2.104) 18-gauge needles are typically used for:
  1. blood donations
  2. routine blood draws
  3. children
  4. small adult veins
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2.105) Which part of a syringe has volume markings such as mL?
  1. Barrel
  2. Flange
  3. Needle
  4. Plunger
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2.106) The gauge size of a needle indicates the diameter of the needle's:
  1. bevel
  2. hub
  3. lumen
  4. shaft
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2.107) What is the phlebotomy device below?
  1. Microtainer tube
  2. Multi-sample blood collection needle
  3. Safety trainer device
  4. Winged infusion set
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2.108) Multisample needles are typically available in which gauges?
  1. 18–20
  2. 20–22
  3. 22–24
  4. 24–26
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2.109) Which part of a needle attaches to the syringe?
  1. Bevel
  2. Hub
  3. Lumen
  4. Shaft
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2.110) Five minutes after a blood draw, the patient is still bleeding from the venipuncture site. What should the phlebotomist do?
  1. Apply a pressure bandage to the patient
  2. Give the patient an anti-inflammatory medication
  3. Notify the patient's nurse
  4. Tell the patient it is okay to leave
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2.111) After use, venipuncture needles should be:
  1. discarded into a sharps container
  2. discarded into an autoclave bag
  3. discarded into the garbage
  4. reused
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2.112) When should you label a blood collection tube?
  1. As soon as you receive the test order
  2. Just before the patient arrives
  3. Before you collect the specimen
  4. After you collect the specimen
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2.113) If a patient is having blood drawn for a prothrombin time test, you might expect:
  1. the patient to be fasting
  2. the patient to bleed for a long time
  3. to have difficulty filling the tube
  4. to have to mix the tube more
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2.114) A phlebotomist has just finished collecting blood samples from a patient. However, the phlebotomist realises she has forgotten to collect a tube. What should she do?
  1. Ask the patient to schedule another appointment
  2. Perform a second collection for the missing tube
  3. Redraw all the tubes
  4. Transfer some blood from a full tube to the empty tube
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2.115) After a blood draw, which of these instructions should you tell the patient?
  1. Bend your arm a few times
  2. Hold the cotton wool in place for 5 minutes
  3. Refrain from drinking fluids for the next 2–3 hours
  4. Walk around if you feel lightheaded
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2.116) Immediately after blood collection, how many times must tubes be inverted to ensure proper mixing of blood and additives?
  1. 1 or 2
  2. 8 to 10
  3. 10 to 15
  4. 15 to 20
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2.117) Why are gauze pads better than cotton balls for holding pressure on the puncture site after venipuncture?
  1. Cotton balls can stick to the puncture site
  2. Cotton balls contain latex which is dangerous for people with latex allergies
  3. Cotton balls have a higher risk of causing a compression nerve injury
  4. Gauze pads are more sterile
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2.118) Patient personal information:
  1. may be discussed during coffee breaks
  2. may be discussed with other patients
  3. may be used for your personal advantage
  4. must never be discussed outside the hospital
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2.119) When interacting with blind patients, you should:
  1. narrate your actions
  2. provide written materials
  3. use an interpreter
  4. use simple language
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2.120) Which zone of interpersonal space does a phlebotomist enter when taking a blood sample?
  1. Intimate
  2. Personal
  3. Public
  4. Social
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2.121) For which type of patient would an interpreter be useful?
  1. Blind
  2. Intellectually disabled
  3. Non-English speaking
  4. Pediatric
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2.122) Plasma is obtained by:
  1. centrifuging a clotted tube
  2. using a red stoppered tube
  3. using a tube containing an anticoagulant
  4. using a tube that does not contain an anticoagulant
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2.123) Pipetting of biological specimens by mouth is:
  1. hazardous and unacceptable
  2. necessary when collecting capillary samples
  3. permissible if one is careful
  4. required when performing serological tests
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2.124) Which anticoagulant is used for red blood cell counts?
  1. EDTA
  2. Heparin
  3. Sodium citrate
  4. Sodium oxalate
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2.125) What is the term for the percentage of red blood cells in a sample of whole blood?
  1. Erythrocyte sedimentation rate
  2. Hematocrit
  3. Hemoglobin
  4. Red blood cell count
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2.126) For a test requiring whole blood, the blood sample must be collected in a vacutainer containing a(n):
  1. anticoagulant
  2. antiglycolytic agent
  3. clot activator
  4. serum separating gel
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2.127) Which routine hematology test includes hemoglobin, hematocrit, red blood count, and white blood count determinations?
  1. CBC
  2. CDC
  3. CPK
  4. CRP
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2.128) Which anticoagulant works by inactivating thrombin?
  1. EDTA
  2. Heparin
  3. Potassium oxalate
  4. Sodium citrate
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2.129) What is the hematocrit?
  1. The average mass of hemoglobin per red blood cell
  2. The rate at which red blood cells descend in a tube
  3. The time it takes for blood to clot
  4. The volume percentage of red blood cells in blood
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2.130) What color vacutainer tube is used to collect blood for blood banking?
  1. Black
  2. Gray
  3. Green
  4. Pink
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2.131) Which medication might cause a patient to bleed excessively from a venipuncture site?
  1. A herbal medicine
  2. An anticoagulant
  3. Birth control pills
  4. Medicine for high cholesterol
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2.132) A member of the clergy is with a patient when you arrive to collect a routine specimen. What should you do?
  1. Ask the patient's nurse what you should do
  2. Come back after the clergy member has gone
  3. Fill out a form saying you were unable to collect the specimen
  4. Interrupt and explain you need to collect a specimen from the patient
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2.133) Which area on a newborn is suitable for skin puncture?
  1. Any calloused areas of the foot
  2. The lateral, flat portion of the heel
  3. The posterior curvature of the heel
  4. The second or third finger on either hand
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2.134) Why is it important to control the depth of the lancet insertion during heel puncture?
  1. To avoid puncturing a vein
  2. To prevent bacterial contamination
  3. To prevent bone injury
  4. To prevent excessive bleeding
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2.135) Which part of an infant's foot is the safest area to perform a capillary puncture?
  1. a
  2. b
  3. c
  4. d
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2.136) What is the maximum lancet size for heel punctures on babies?
  1. 1 mm
  2. 2 mm
  3. 3 mm
  4. 4 mm
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2.137) Which specimen is used for coagulation testing?
  1. Plasma
  2. Serum
  3. Urine
  4. Whole blood
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2.138) Which sample does the glycosylated hemoglobin test require?
  1. Plasma
  2. Serum
  3. Urine
  4. Whole blood
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2.139) Therapeutic phlebotomy is the preferred treatment for:
  1. hemochromatosis
  2. hemophilia
  3. myeloma
  4. thalassemia
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2.140) Which of these statements about the blood gas test is true?
  1. The specimen is collected in a plain red top tube
  2. The specimen is frozen immediately after collecting
  3. The specimen is tested within 24 hours
  4. The test requires an arterial sample
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2.141) To perform an arterial puncture, you need heparin solution, sterile gauze, betadine wipes, a sterile needle, a syringe and:
  1. a butterfly needle
  2. a tourniquet
  3. alcohol wipes
  4. ice water
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2.142) You are taking an arterial puncture. What is the first area of the patient’s body you should examine for a suitable site?
  1. The antecubital area of both arms
  2. The brachial artery
  3. The femoral artery
  4. The thumb side of the wrist
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2.143) Which of these is an acceptable needle gauge for an arterial puncture?
  1. 10
  2. 18
  3. 20
  4. 28
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2.144) When performing a radial artery puncture, the needle should enter the skin:
  1. 1 cm distal to the index finger
  2. 1 cm lateral to the index finger
  3. at the exact point where the pulse is felt
  4. proximal to where the pulse is felt
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2.145) Which of these is a patient identifier?
  1. Date of collection
  2. Patient name
  3. Specimen type
  4. Time of collection
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2.146) PKU tests are performed routinely on:
  1. HIV patients
  2. cancer patients
  3. new employees
  4. newborn infants
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2.147) A phlebotomist has mixed up two specimens. What should the phlebotomist do?
  1. Ask the nurse to identify the specimen
  2. Ask the patients which one is theirs
  3. Continue regardless
  4. Retake both specimens
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2.148) An ABG test measures the amount of:
  1. bacteria and yeast in a urine sample
  2. occult blood in a stool sample
  3. oxygen and carbon dioxide in arterial blood
  4. red and white blood cells in venous blood
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2.149) 2-hr PC means 2 hours:
  1. after a meal
  2. after an operation
  3. before a meal
  4. before an operation
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2.150) An inpatient is eating breakfast when you arrive to collect a specimen for a fasting blood glucose test. Which of these would be the best action to take?
  1. Ask the patient to vomit into a sink or emesis basin before taking the sample
  2. Ask the patient's nurse if the specimen should still be collected
  3. Draw the specimen quickly before the patient finishes eating
  4. Give the patient a dose of insulin to counteract the food they have eaten
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2.151) Which of the following tube additives is preferred for the collection of a blood culture specimen?
  1. Citrate phosphate dextrose
  2. Potassium oxalate
  3. Sodium heparin
  4. Sodium polyanethol sulfonate
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2.152) Which of these is the most important part of blood culture collection?
  1. Adequately filling two media vials
  2. Asepsis of the collection site
  3. Selecting the collection site
  4. Timing of the second set of cultures
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2.153) It is 7:15 pm and you must draw a blood culture from a patient who is receiving a course of antibiotics. His next doses of antibiotics are due at 10 pm and 6 am. When is the best time to draw the blood culture?
  1. At 2 am
  2. At midnight
  3. Just after his next dose
  4. Just before his next dose
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2.154) How many bottles are drawn for a blood culture set?
  1. 1
  2. 2
  3. 3
  4. 4
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2.155) A patient begins a routine glucose tolerance test. After 30 minutes, the patient's blood sugar is 374 mg/dL. What should you do?
  1. Do nothing and continue with the test
  2. Make a note and continue with the test
  3. Start the test again
  4. Tell the attending nurse immediately
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2.156) What is the minimum number of blood samples needed for the one-step glucose tolerance test?
  1. 1
  2. 2
  3. 3
  4. 4
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2.157) You are performing a glucose tolerance test that requires a blood sample at baseline and then four blood samples thereafter, at a rate of one sample every 30 minutes. If the patient drinks the glucose at 10:00 am, then at what time should you take the final blood sample?
  1. 11:00 am
  2. 10:30 am
  3. 11:30 am
  4. 12:00 pm
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2.158) Why should capillary puncture never be performed on an infant’s finger?
  1. It could cause excessive blood loss
  2. It could damage bones and nerves
  3. The blood in the finger does not represent blood in the rest of the body
  4. There is a high risk the infant will move their finger during the puncture
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2.159) Why are finger punctures made at right angles to fingerprint striations?
  1. To facilitate blood sample collection
  2. To prevent excessive bleeding
  3. To prevent scar formation
  4. To reduce pain
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2.160) You puncture a patient's finger for a finger stick blood test but no blood comes out. What should you do?
  1. Cool the finger using an ice pack
  2. Puncture the site again
  3. Scrape the puncture site against the test strip
  4. Squeeze the finger
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2.161) The glycosylated hemoglobin test is used to diagnose which disease?
  1. Diabetes
  2. Hemoglobin C disease
  3. Inflammation
  4. Renal disease
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Section 3: Specimen handling, transport, and processing

3.1) Sodium fluoride preserves:
  1. cellulite
  2. cellulose
  3. glucose
  4. glycerin
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3.2) What can happen if blood specimen tubes are not adequately mixed with anticoagulant?
  1. Clotting
  2. Hemolysis
  3. Hyperglycemia
  4. Leukocytosis
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3.3) A phlebotomist draws blood from a patient and then wraps the tube in aluminum foil. What analyte is being tested?
  1. Albumin
  2. Bilirubin
  3. PTT
  4. Urobilinogen
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3.4) Which pipette has a bulged-out portion in the middle?
  1. Mohr
  2. Pasture
  3. Serological
  4. Volumetric
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3.5) Why does volumetric glassware have the letters TC inscribed on it?
  1. Because it can hold toxic chemicals
  2. Because it contains a fixed volume
  3. Because it delivers a fixed volume
  4. Because it is Type C glassware
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3.6) The icterus index is a measure of which color of serum?
  1. Green
  2. Red
  3. White
  4. Yellow
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3.7) What color is lipemic plasma?
  1. Brownish-yellow
  2. Clear red
  3. Milky
  4. Straw-colored
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3.8) While separating serum, you notice that it is yellow, indicating that it contains excess bilirubin. What is the term for this finding?
  1. Hemolysis
  2. Icterus
  3. Intergeneric
  4. Lipemia
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3.9) A buffy coat forms when:
  1. an SST is left to rest for 24 hours
  2. whole anticoagulated blood is centrifuged
  3. whole blood is left to rest for 24 hours
  4. whole coagulated blood is centrifuged
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3.10) Which of these specimens is unacceptable for testing?
  1. A blood specimen collected for coagulation testing in a citrate tube
  2. A blood specimen in an EDTA tube that is three-quarters full
  3. A clotted blood specimen for HbA1c testing
  4. A sputum specimen containing 52 leukocytes and 5 epithelial cells per LPF
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3.11) Which of these specimens would most likely be rejected for testing?
  1. A hemolyzed potassium specimen
  2. A nonfasting glucose specimen
  3. A serum-separator tube that is only half-filled
  4. An icteric bilirubin specimen
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3.12) When a blood specimen in a gray-top tube is centrifuged, what does it separate into?
  1. Plasma, buffy coat and red blood cells
  2. Plasma, buffy coat and white blood cells
  3. Serum, buffy coat and red blood cells
  4. Serum, buffy coat and white blood cells
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3.13) What is a buffy coat made of?
  1. Granulocytes and fibrin
  2. Red blood cells and granulocytes
  3. Reticulocytes and granulocytes
  4. White blood cells and platelets
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3.14) Which device measures the RPM of a centrifuge?
  1. Ohmmeter
  2. Rheostat
  3. Tachometer
  4. Voltmeter
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3.15) What is the most common cause of excessive centrifuge vibration?
  1. Electrical interference
  2. Unbalanced tubes
  3. Uneven bench surfaces
  4. Variable voltage
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3.16) For how many minutes should a microhematocrit be centrifuged?
  1. 1
  2. 2
  3. 5
  4. 15
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3.17) What is the most important rule about centrifuges?
  1. Always balance the tubes placed inside
  2. Make sure to set the rheostat at 3000 rpm for the top speed
  3. Make sure to set the timer
  4. Never remove the rubber cushions in the cups
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3.18) Which of the following can cause hemolysis in a blood sample?
  1. Collecting the sample in a serum separator tube
  2. Shaking the sample vigorously
  3. Using a large needle
  4. Withdrawing the needle before removing the tourniquet
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3.19) Which sample is needed for a cardiac enzyme test?
  1. Blood
  2. Sputum
  3. Sweat
  4. Urine
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3.20) The creatinine clearance test:
  1. is a liver function test
  2. requires a blood sample and a 24-hour urine collection
  3. requires the patient to be fasting at the onset of testing
  4. requires timed blood samples to be drawn
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3.21) Which of these tests is the most likely to require chain-of-custody documentation?
  1. Blood culture
  2. Cross-match
  3. Drug screen
  4. TDM
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3.22) The alpha-fetoprotein (AFP) test requires which type of sample?
  1. Blood
  2. Cerebrospinal fluid
  3. Feces
  4. Urine
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3.23) Which of these tests would most be affected if a patient ate a muffin and orange juice before a fasting blood test?
  1. Blood culture
  2. Cardiac enzymes
  3. Complete blood count
  4. Glucose
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3.24) What is the purpose of a custody seal on a sample container?
  1. To help identify the sample if the container is misplaced
  2. To prevent air from entering the container
  3. To show whether the container has been tampered with
  4. To show who currently has custody of the container
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3.25) Which of the following is NOT required on a chain of custody form?
  1. The name of the patient's doctor
  2. The name of the person who collected the specimen
  3. The time the specimen was collected
  4. The type of specimen collected
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3.26) You receive a specimen that should come with a chain of custody form. However, the form is missing. What should you do?
  1. Ask for a new specimen to be collected
  2. Fill out a new chain of custody form yourself
  3. Perform the test anyway
  4. Use a chain of custody form from a specimen of a different patient
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3.27) Which of these tests requires chain of custody documentation?
  1. Complete blood count
  2. Fetal fibronectin
  3. Free light chains test
  4. Legal paternity test
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3.28) A courier delivers a chain-of-custody sample to a technician at a laboratory. Who needs to sign and date the chain-of-custody form?
  1. Only the courier
  2. Only the laboratory technician
  3. The courier and the laboratory technician
  4. The laboratory manager and the laboratory technician
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3.29) A blood sample of insufficient quantity would be considered:
  1. QA
  2. QC
  3. QLA
  4. QNS
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3.30) Which of these tests is centrifuged?
  1. Blood gas
  2. Blood smear
  3. Complete blood count
  4. Hematocrit
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3.31) What of these is a sign of hemolyzed plasma?
  1. Air bubbles
  2. Blood clots
  3. Increased opacity
  4. Red color
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3.32) A fasting patient has cloudy white serum. What condition does the patient have?
  1. Hemolysis
  2. Hyperbilirubinemia
  3. Hyperthyroidism
  4. Hypertriglyceridemia
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3.33) What is an aliquot?
  1. A dilution of a sample
  2. A smaller amount taken from an original specimen
  3. The leftover unusable part of a sample after testing
  4. The minimum amount of sample required for a specific test
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3.34) Blood specimens for which of these tests require cooling during transport?
  1. Blood culture
  2. Cold agglutinin
  3. Cryoglobulin
  4. Homocysteine
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3.35) Blood samples for complete blood counts must be transported:
  1. in a Styrofoam-lined box with dry ice
  2. in a vacuum-sealed container at room temperature
  3. in an insulated container with a cold pack
  4. wrapped in metal foil
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3.36) Blood samples for ammonia tests are transported on ice to prevent the:
  1. breakdown of ammonia
  2. conversion of ammonia to nitrite
  3. generation of ammonia
  4. growth of pathogenic microorganisms
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3.37) At what temperature are cryofibrinogen specimens transported?
  1. −20°C or lower
  2. 4–6°C
  3. 21°C
  4. 37°C
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3.38) Blood specimens for sodium testing must be transported at which temperature?
  1. –20°C
  2. 1–6°C
  3. 20–24°C
  4. 37–40°C
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3.39) What is the purpose of activated charcoal in transport media?
  1. To absorb toxic substances
  2. To kill non-pathogens
  3. To maintain a neutral pH
  4. To prevent the overgrowth of extraneous organisms
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3.40) Which of these biochemistry tests should be kept away from the light?
  1. Bilirubin
  2. Calcium
  3. Glucose
  4. Urea
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3.41) Where is blood generally stored?
  1. At room temperature
  2. In a water bath
  3. In the freezer
  4. In the refrigerator
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3.42) Blood samples for cold agglutinin testing should be kept at what temperature before testing?
  1. –6°C
  2. 6°C
  3. 20°C
  4. 37°C
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3.43) Serum specimens for vitamin K should be:
  1. kept at 37–40°C
  2. kept away from light
  3. mixed with an antiglycolytic agent
  4. mixed with glass beads
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3.44) Hemolysis of a blood specimen would NOT affect which test result?
  1. Haptoglobin
  2. MCHC
  3. Red blood cell count
  4. Total hemoglobin
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3.45) When a blood sample is left at room temperature, which of these increases?
  1. Carbon dioxide
  2. Glucose
  3. Hemoglobin
  4. Potassium
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3.46) The results of which of these tests may be falsely increased if the stopper is removed from the specimen?
  1. Cortisol
  2. Glucose
  3. Potassium
  4. pH
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3.47) Extra precautions are necessary when labeling specimens for:
  1. C&S
  2. blood typing
  3. factor assays
  4. therapeutic drug monitoring
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3.48) The Herpes B virus and Ebola virus must be transported as:
  1. Category A
  2. Category B
  3. genetically modified organisms
  4. solid medical waste
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3.49) When shipping infectious substances, where should you write the technical name of the infectious agent?
  1. On the Shipper’s Declaration
  2. On the air waybill
  3. On the outside packaging
  4. On the primary container
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3.50) Specimens containing the smallpox virus must be transported as:
  1. Category A
  2. Category B
  3. contagious specimen
  4. infectious specimen
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3.51) Infectious substances are part of which class in the Transportation of Dangerous Goods Act?
  1. 2
  2. 3
  3. 5
  4. 6
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3.52) What packaging is used to ship Category A infectious substances?
  1. A610
  2. A630
  3. P620
  4. P650
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3.53) Infectious substances that pose the highest risk of infection during transportation and are capable of causing permanent disability, life-threatening disease, or fatal disease are known as ______________ substances.
  1. Category A
  2. Category B
  3. Category C
  4. Type 1B
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3.54) Category B infectious substances must be shipped with a label that says:
  1. UN2814 – Infectious Substance, Category B, Affecting Humans
  2. UN3291 – Clinical Waste, Category B
  3. UN3373 – Biological Substance, Category B
  4. UN3549 – Medical Waste, Category B, Affecting Humans
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Section 4: Waived and point-of-care-testing

4.1) Which of these biomarkers can urine refractometers measure?
  1. The presence of nitrates in urine
  2. Urine hemoglobin concentration
  3. Urine osmolality
  4. Urine specific gravity
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4.2) The bleeding time test assesses the function of which cells?
  1. Eosinophils
  2. Lymphocytes
  3. Platelets
  4. Red blood cells
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4.3) How often should you blot the blood during a bleeding time test?
  1. Every 10 seconds
  2. Every 30 seconds
  3. Every minute
  4. Every 2 minutes
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4.4) Which test evaluates the extrinsic pathway of coagulation?
  1. Closure time
  2. Partial thromboplastin time
  3. Prothrombin time
  4. Thrombin time
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4.5) The Platelet Function Analyzer-100 (PFA-100) requires:
  1. EDTA whole blood
  2. citrated whole blood
  3. heparinized whole blood
  4. plasma
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4.6) The partial thromboplastin time (PTT) test requires what type of blood sample?
  1. Coagulated
  2. Decalcified
  3. Defibrinated
  4. Frozen
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4.7) In the prothrombin time test, the patient's plasma is mixed with:
  1. calcium and activator
  2. calcium and thromboplastin
  3. glass beads
  4. platelet lipids
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4.8) Pregnancy test strips detect the presence of which hormone?
  1. Adrenocorticotropic hormone
  2. Estrogen
  3. Human chorionic gonadotropin
  4. Progesterone
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4.9) What area should you swab to obtain a throat culture?
  1. Larynx
  2. Oral mucosa
  3. Pharynx
  4. Trachea
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4.10) According to CDC guidelines, how far back should you tilt the patient’s head when performing a nasopharyngeal swab for COVID-19 testing?
  1. 20 degrees
  2. 45 degrees
  3. 70 degrees
  4. 100 degrees
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4.11) A swab must be inserted how far into the nasal cavity to reach the nasopharynx?
  1. 3–4 cm
  2. 5–6 cm
  3. 7–8 cm
  4. 9–10 cm
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4.12) Which sample does the rapid strep test require?
  1. Nasal swab
  2. Nasopharyngeal swab
  3. Throat swab
  4. Vaginal swab
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4.13) There are two reasons why phlebotomists should wipe away the first drop of blood when performing a capillary puncture. The first reason is to remove traces of alcohol. What is the second reason?
  1. The first drop of blood contains excess tissue fluid
  2. The first drop of blood is high in clotting factors
  3. To increase blood flow to the area
  4. To remove pathogens
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4.14) Which of these statements about capillary punctures is true?
  1. It is better to clean the skin with iodine than with alcohol
  2. The first drop of blood is the best to use
  3. The puncture must hit bone for it to be deep enough
  4. The puncture should be made at right angles to the fingerprint striations
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4.15) Which is the preferred site to obtain capillary blood from adults?
  1. Ear
  2. Fingertip
  3. Heel
  4. Toe
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4.16) The middle finger and ___________ are used for finger puncture.
  1. index finger
  2. little finger
  3. ring finger
  4. thumb
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4.17) What is the main reason a capillary puncture site is warmed beforehand?
  1. To delay clotting
  2. To increase blood flow
  3. To minimize contamination
  4. To reduce hemoconcentration
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4.18) Which of these tests is collected first during a single capillary puncture?
  1. Complete blood count
  2. Electrolytes
  3. Glucose
  4. Phosphorus
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4.19) What chemical does the urine glucose dipstick test use?
  1. Glucose oxidase
  2. Glucose oxide
  3. Glucose reductase
  4. Hexichloridine
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4.20) Which of these actions is NOT performed when performing quality control of a glucometer?
  1. Apply a drop of blood to the test strip
  2. Compare the result displayed by the glucometer with the expected value
  3. Insert a test strip into the glucometer
  4. Remove the test strip from the glucometer
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4.21) Glucose point-of-care testing is used to screen for:
  1. glycemia
  2. glycogenolysis
  3. glycolysis
  4. glycosuria
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4.22) Which of these is a CLIA waived test?
  1. Complete blood count
  2. Fecal occult blood test
  3. Immunoassay
  4. Peripheral smear
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4.23) When a person tests negative for COVID-19 with a rapid antigen test but is still suspected of having the virus, they should be tested with a(n):
  1. ELISA
  2. PCR test
  3. antibody test
  4. lateral flow test
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Section 5: Non-blood specimens

5.1) Which of these fluids is normally clear and colorless?
  1. Cerebrospinal fluid
  2. Lymph fluid
  3. Serum
  4. Urine
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5.2) To reach the urinary bladder, urine passes from the kidney through a tube called the:
  1. renal column
  2. renal pelvis
  3. ureter
  4. urethra
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5.3) In the kidneys, substances travel from the glomerulus into the Bowman capsule by which process?
  1. Active transport
  2. Diffusion
  3. Filtration
  4. Osmosis
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5.4) What is the main site for the absorption of digested food?
  1. Kidneys
  2. Large intestine
  3. Small intestine
  4. Stomach
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5.5) What is the functional unit of the lungs?
  1. Alveoli
  2. Lobule
  3. Nephron
  4. Neuron
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5.6) Which of these chemicals would be the best choice to neutralize a weak acid spill in the laboratory?
  1. Acetic acid
  2. Boric acid
  3. Sodium bicarbonate
  4. Sodium hydroxide
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5.7) Stool specimens for O&P should be collected in a jar containing:
  1. SAF fixative
  2. agar
  3. saline
  4. sodium hypochlorite
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5.8) The sweat test is used to diagnose which disease?
  1. Cystic fibrosis
  2. Infection
  3. Muscular dystrophy
  4. Pneumonia
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5.9) Which of these foods should a patient avoid eating before a fecal occult blood test?
  1. Fish
  2. Popcorn
  3. Red meat
  4. Whole wheat bread
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5.10) Throat swabs are often taken to diagnose:
  1. bleeding ulcers
  2. herpes zoster
  3. streptococcus
  4. typhoid fever
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5.11) Which organ does hydrogen breath testing investigate?
  1. Intestine
  2. Liver
  3. Lungs
  4. Stomach
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5.12) The H. pylori breath test detects which gas?
  1. Carbon dioxide
  2. Hydrogen
  3. Nitrogen
  4. Oxygen
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5.13) What sample is needed for the Hemoccult test?
  1. Blood
  2. Sputum
  3. Stool
  4. Urine
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5.14) Urine, breath, and _______ are used for ketone testing.
  1. blood
  2. semen
  3. stool
  4. sweat
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5.15) How often should reagent strips be tested with controls?
  1. Daily
  2. Weekly
  3. Monthly
  4. Every three months
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5.16) Identify the false statement regarding 24-hour urine collection.
  1. A preservative may be used for some tests
  2. Collections during the night are discarded
  3. Testing is done in clinical chemistry
  4. The first timed specimen is discarded
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5.17) When used for detecting urinary tract infections, urine dipsticks test for:
  1. nitrates
  2. nitrites
  3. phosphates
  4. sugars
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5.18) A patient tests positive for bilirubin on a urine strip. Which test can rule out a false positive result?
  1. Acetest
  2. Clinitest
  3. Ictotest
  4. SSA
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5.19) Urine samples for culture should be collected in containers that:
  1. are narrow-mouthed
  2. are sterile
  3. contain an anticoagulant
  4. have been cleaned with disinfectant
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5.20) Midstream urine samples are most often used for:
  1. culture and susceptibility
  2. mononucleosis testing
  3. pregnancy testing
  4. routine urinalysis
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5.21) Clean-catch urine specimens are necessary when testing urine for:
  1. bacteria
  2. glucose
  3. occult blood
  4. protein
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5.22) Which urine collection technique involves collecting the urine by inserting a sterile needle into the patient's bladder through the abdominal wall?
  1. Biopsy
  2. Necropsy
  3. Suprapubic aspiration
  4. Urinary catheter
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5.23) Which of these statements about 24-hour urine collections is true?
  1. If the container contains a preservative, then the preservative must be thrown away
  2. If the patient leaves their house, they should take the container with them
  3. Night-time specimens are discarded
  4. The patient should keep the container in a warm place
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5.24) What preservative is needed for a sodium 24-hour urine test?
  1. Chlorhexidine
  2. HCl
  3. NaHCO₃
  4. No preservative
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5.25) Which of these media is used to transport and preserve stool specimens?
  1. Blood agar
  2. Cary-Blair media
  3. Lowenstein-Jensen agar
  4. MacConkey agar
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5.26) Which of these urine constituents increases in a urine specimen left at room temperature?
  1. Bilirubin
  2. Leukocytes
  3. Nitrites
  4. Urobilinogen
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5.27) Which of these chemicals can be used to preserve 24-hour urines for endocrine testing?
  1. Boric acid
  2. Potassium chloride
  3. Sodium bicarbonate
  4. Sodium hypochlorite
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5.28) Which of these specimens needs to be kept at 35–37°C?
  1. Blood culture
  2. Feces
  3. Throat swab
  4. Urine
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5.29) Which of these specimens is sterile in healthy people?
  1. Blood
  2. Feces
  3. Gastrointestinal tract specimens
  4. Sputum
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5.30) An elevated level of white blood cells in urine is called:
  1. hematuria
  2. pyelonephritis
  3. pyuria
  4. uremia
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5.31) Before you perform urinalysis on a refrigerated urine specimen, bring the urine to room temperature and _______ it.
  1. boil
  2. centrifuge
  3. filter
  4. stir
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5.32) What do the letters TCA on a urine drug test stand for?
  1. Target channel adapter
  2. Trichloroacetic acid
  3. Trichloroethane
  4. Tricyclic antidepressant
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5.33) Amorphous urates:
  1. disappear if the urine is heated
  2. form blue sediment in acidic urine
  3. form white sediment in alkaline urine
  4. have a non-granular appearance
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5.34) What color is the urine of patients with jaundice?
  1. Brownish-yellow
  2. Milky
  3. Red
  4. Straw-colored
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5.35) Bile pigments will cause urine specimens to be what color?
  1. Cloudy
  2. Foul-smelling
  3. Red
  4. Yellow-brown
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5.36) A patient's urine sample is positive for ketones. The patient probably has:
  1. advanced liver disease
  2. heart disease
  3. pernicious anemia
  4. uncontrolled diabetes
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5.37) What is the SI unit for reporting the result of urine glucose tests?
  1. mg/L
  2. mg/dL
  3. mmol/L
  4. mmol/dL
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5.38) Which of these tests does NOT require a 24-hour urine specimen?
  1. Creatinine clearance
  2. Ketones
  3. Ketosteroids
  4. Quantitative drug analysis
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5.39) Which of these would be checked during a macroscopic examination of urine?
  1. Acidity
  2. Appearance under a microscope
  3. Color
  4. Specific gravity
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5.40) A urine's specific gravity is directly proportional to its:
  1. dissolved solids
  2. salt content
  3. sugar content
  4. turbidity
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5.41) What are hyaline casts made of?
  1. Albumin
  2. Globulin
  3. Immunoglobulins G and M
  4. Uromodulin
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5.42) Urine that smells like ammonia may be a sign the patient has:
  1. a Proteus infection
  2. a yeast infection
  3. diabetes
  4. hepatitis
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5.43) Which epithelial cells in urine sediment are the largest?
  1. Columnar
  2. Cuboidal
  3. Squamous
  4. Urothelial
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5.44) Which type of urinary cast is a sign of chronic renal failure?
  1. Fatty
  2. Renal tubular epithelial cell
  3. Waxy
  4. White blood cell
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5.45) Which test uses guaiac paper?
  1. Fecal occult blood test
  2. Helicobacter pylori breath test
  3. Ketones urine test
  4. Urea breath test
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5.46) A pH of 8 is:
  1. acidic
  2. alkaline
  3. negative
  4. neutral
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Section 6: Laboratory operations

6.1) A technique that has demonstrated its superiority over other techniques over time is known as the:
  1. Pareto principle
  2. benchmark
  3. best practice
  4. case model
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6.2) What is the term for mechanisms and processes protecting health information from unauthorized access?
  1. Accountability
  2. Confidentiality
  3. Isolation
  4. Security
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6.3) When talking to children, use:
  1. abbreviations
  2. jargon
  3. simple words
  4. technical language
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6.4) When communicating with someone from a different culture, it is important to:
  1. make assumptions about their beliefs and values
  2. speak louder and slower to ensure understanding
  3. be respectful of their cultural differences
  4. avoid using humor, as it may be misinterpreted
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6.5) Which of these is one of the four principles of the “four principles plus scope” approach to medical ethics?
  1. Integrity
  2. Justice
  3. Professionalism
  4. Sensitivity
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6.6) A patient rolls up his sleeve for a blood draw. Which type of consent is this?
  1. Active
  2. Expressed
  3. Implied
  4. Informed
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6.7) Which acceptable range do laboratories typically use for quality control results?
  1. One standard deviation from the mean
  2. The middle 68% of the results
  3. The outer 5% of the results
  4. Two standard deviations from the mean
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6.8) In which of these situations should a lab technician remove their gloves?
  1. Before carrying specimens through a designated clean area
  2. Before loading the autoclave
  3. Before using a restroom
  4. For cleaning a chemical spill
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6.9) Which link in the chain of infection is a skin cut?
  1. Mode of transmission
  2. Portal of entry
  3. Reservoir
  4. Susceptible host
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6.10) Used needles should be discarded in:
  1. a biohazard sharps container
  2. a glass disposal bucket
  3. a glass recycling bin
  4. the regular garbage
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6.11) Class C fires involve:
  1. combustible metals
  2. electrical equipment
  3. flammable liquids
  4. paper and wood
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6.12) What is the first step when using a fire extinguisher?
  1. Aim the fire extinguisher at the fire
  2. Pull the pin
  3. Squeeze the handle
  4. Sweep the hose from side to side at the base of the fire
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6.13) What type of fire extinguisher is used on class D fires?
  1. CO2
  2. Dry powder
  3. Foam
  4. Water
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6.14) A fire extinguisher with a 6A-80BC rating can put out ________________ fire as a 3A-40BC extinguisher.
  1. half as much
  2. the same amount of
  3. three times as much
  4. twice as much
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6.15) A fire extinguisher has the following symbol: The fire extinguisher:
  1. can be used to extinguish electrical fires
  2. cannot be used on electrical equipment
  3. carries a risk of electric shock
  4. requires charging before use
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6.16) A fire extinguisher has the following symbol: This fire extinguisher:
  1. can be used on fires involving flammable liquids or gases
  2. requires annual top-ups of type B fuel
  3. should be stored in a separate room from flammable chemicals
  4. uses kerosene to extinguish fires
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6.17) The fire extinguisher below can be used on which of the following fires?
  1. Electrical equipment
  2. Methanol
  3. Oil bath
  4. Cardboard boxes
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6.18) What are the three components of the fire triangle?
  1. Oxygen, heat, and fuel
  2. Oxygen, ignition, and fuel
  3. Rescue, Alarm, Escape
  4. Rescue, Alarm, Extinguish
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6.19) Which of these chemicals is corrosive to skin?
  1. Calcium acetate
  2. Hydrogen peroxide
  3. Potassium phosphate
  4. Sodium chloride
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6.20) What is the term for a chemical that immediately damages human tissue on contact?
  1. Carcinogenic
  2. Combustible
  3. Corrosive
  4. Toxic
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6.21) What is the term for chemicals that cause birth defects in embryos and fetuses?
  1. Carcinogens
  2. Corrosives
  3. Phenyls
  4. Teratogens
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6.22) Which of these is written information that must be supplied by manufacturers of chemicals and hazardous materials?
  1. OSHA
  2. OSM
  3. SDS
  4. TOSM
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6.23) What is the term for chemicals that burn or destroy tissue?
  1. Acyclic
  2. Caustic
  3. Galvanic
  4. Stereogenic
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6.24) Mixing bleach with acid produces water, salt and:
  1. arsenic trioxide
  2. chlorine gas
  3. phosgene
  4. sodium cyanide
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6.25) By which route do toxic chemicals enter the body by direct skin contact?
  1. Absorption
  2. Ingestion
  3. Inhalation
  4. Injection
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6.26) Which type of glove has the highest resistance to permeation by gases?
  1. Butyl
  2. Neoprene
  3. PVC
  4. Rubber
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6.27) When visiting a patient in enteric isolation, staff must wear gloves and what other item of equipment?
  1. Gown
  2. Hazard suit
  3. Helmet
  4. Shoe covers
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6.28) What is the correct order of removal of PPE?
  1. Gloves, gown, goggles, mask
  2. Gown, goggles, gloves, mask
  3. Gown, mask, gloves, goggles
  4. Mask, gown, gloves, goggles
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6.29) Which of these items is PPE?
  1. Biohazard bag
  2. Countertop splash shield
  3. Latex gloves
  4. Sharps container
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6.30) A substance with the potential to produce cancer in humans or animals is called a:
  1. carcinogen
  2. caustic substance
  3. nosocomial substance
  4. pathogenic substance
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6.31) A wavelength of 250 nm falls into which category of electromagnetic radiation?
  1. Infrared
  2. Radio waves
  3. Ultraviolet
  4. Visible
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6.32) The three basic protective measures against radiation are time, distance, and:
  1. lead
  2. radiation suit
  3. shielding
  4. speed
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6.33) Universal precautions are based on what assumption?
  1. All laboratory chemicals may be carcinogenic
  2. All specimens are potentially infectious
  3. Equipment is not sterile
  4. Handwashing is the best way to prevent the spread of infection
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6.34) What does this pictogram mean?
  1. Flammable
  2. Oxidizing
  3. Self-heating
  4. Self-reactive
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6.35) What does this pictogram mean?
  1. Biohazard
  2. Carcinogenic
  3. Corrosive
  4. Toxic
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6.36) What does the following sign mean?
  1. Biohazard
  2. Do not eat
  3. General warning
  4. Toxic material
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6.37) In the NFPA hazard diamond, which quadrant indicates special hazards?
  1. Blue
  2. Red
  3. White
  4. Yellow
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6.38) On the NFPA hazard diamond, a number 1 in the red quadrant means the substance:
  1. does not burn
  2. only ignites after considerable preheating
  3. ignites if moderately heated
  4. can be ignited at normal temperatures
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6.39) Always keep picric acid:
  1. dry
  2. refrigerated
  3. ventilated
  4. wet
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6.40) Which of these dilutions is the most effective disinfectant?
  1. 1% ethyl alcohol
  2. 10% ethyl alcohol
  3. 40% ethyl alcohol
  4. 70% ethyl alcohol
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6.41) What is the first step when cleaning up a blood spill?
  1. Decontaminate reusable equipment such as dustpans and brushes
  2. Pour disinfectant onto the spill
  3. Put on PPE
  4. Soak up the spill with paper towels
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6.42) Which concentration of bleach is used to decontaminate benchtops?
  1. 10%
  2. 20%
  3. 30%
  4. 50%
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6.43) A disinfectant may become ineffective if:
  1. it is kept in a dark place
  2. it is left at room temperature
  3. it is not diluted properly
  4. the container is kept tightly sealed during storage
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6.44) Which disinfectant inactivates prions?
  1. Alcohol
  2. Formaldehyde
  3. Formalin
  4. Sodium hydroxide
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6.45) Which of these substances would be the best solution to clean up a large blood spill?
  1. 1:10 dilution of bleach
  2. 70% isopropyl alcohol
  3. Antibacterial soap and water
  4. Povidone-iodine
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6.46) At what temperature do hot air ovens sterilize glassware in 60 minutes?
  1. 100°C
  2. 121°C
  3. 140°C
  4. 160°C
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6.47) Which pathogen cannot be inactivated by conventional sterilization methods such as autoclaving, 70% ethanol, UV disinfection, and gamma irradiation?
  1. Coccidioides immitis
  2. Mycobacterium tuberculosis
  3. Prions
  4. Smallpox
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6.48) Preventive maintenance means:
  1. fixing things before they break
  2. identifying unexpected failures
  3. repairing equipment after it fails
  4. turning off machines when they are not in use
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6.49) Which glassware is used to measure 24-hour urine volumes?
  1. Beaker
  2. Erlenmeyer flask
  3. Graduated cylinder
  4. Volumetric flask
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6.50) What type of filter do biological safety cabinets use to remove airborne particles, such as bacteria and viruses?
  1. Electrostatic
  2. Fiberglass
  3. HEPA
  4. UV light
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6.51) What virus causes hepatitis C?
  1. HAV
  2. HBC
  3. HCV
  4. HPC
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6.52) The focus of quality assurance is best described as:
  1. continuous quality improvement
  2. doing more with less
  3. improving a system
  4. preventing mistakes and defects
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6.53) What is the purpose of utilization management?
  1. To improve the quality of patient care
  2. To increase patient safety
  3. To reduce the number of adverse events
  4. To use resources more effectively
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6.54) Which of these is a pre-analytical error?
  1. A light blue top tube filled only halfway
  2. A result reported on the wrong patient
  3. Incorrect calculation of test results
  4. Incorrect interpretation of results
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6.55) The process of checking and rectifying the precision and accuracy of a measuring instrument is called:
  1. calibration
  2. estimation
  3. graduation
  4. mensuration
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6.56) Which of these practices is dangerous and should be avoided?
  1. Immediately performing first aid to someone who has been exposed to blood
  2. Recapping used needles using both hands
  3. Securing used sharps containers during transport
  4. Using plasticware instead of glass
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6.57) You are a phlebotomist drawing blood from a woman for a pregnancy test. After the woman has left the room, a colleague approaches you and asks what test was ordered. He explains he wants to know because he is the woman's ex-boyfriend. You should:
  1. call the police
  2. inform him you are unable to tell him anything
  3. lie and tell him the test was a complete blood count
  4. reassure him that he has nothing to worry about
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6.58) When shipping infectious organisms, in which shape must the marking UN3373 appear on the box?
  1. Circle
  2. Diamond
  3. Hexagon
  4. Triangle
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6.59) Under the Occupational Safety and Health Act, employers have a responsibility to provide:
  1. a safe workplace
  2. extra training for disabled workers
  3. health insurance
  4. work breaks
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6.60) If a worker suffers an accident at work and has to be hospitalized, how soon must the employer notify OSHA?
  1. Immediately
  2. Within 12 hours
  3. Within 24 hours
  4. Within 8 hours
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6.61) In OSHA's hierarchy of controls, what is the most effective type of hazard control?
  1. Administrative controls
  2. Elimination
  3. Engineering controls
  4. Substitution
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6.62) OSHA's Hazard Communication standard requires employers to inform workers about the workplace dangers of:
  1. bloodborne pathogens
  2. dangerous chemicals
  3. general safety hazards
  4. miscommunication
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6.63) What is medical negligence?
  1. A situation where an injured patient has no evidence of how the injury occurred
  2. An agreement for a doctor to provide treatment
  3. The failure to provide a patient with a reasonable standard of care
  4. The submission of insurance claims for services never provided
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6.64) Another term for professional negligence is:
  1. malfeasance
  2. malpractice
  3. misfeasance
  4. nonfeasance
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6.65) The 4 D's of medical negligence are duty, dereliction, direct causation, and:
  1. damages
  2. danger
  3. denial
  4. doctor
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6.66) Which legal term means the degree of care a prudent, reasonable healthcare provider would exercise in a given situation?
  1. Customary care
  2. Optimal care
  3. Scope of practice
  4. Standard of care
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6.67) What federal agency monitors the Occupational Safety and Health Act?
  1. Environmental Protection Agency (EPA)
  2. Food and Drug Administration (FDA)
  3. Occupational Safety and Health Administration (OSHA)
  4. United States Public Health Service (USPHS)
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6.68) What federal agency categorizes clinical laboratory tests by their complexity?
  1. Centers for Disease Control and Prevention (CDC)
  2. Clinical Laboratory Improvement Amendments (CLIA)
  3. Food and Drug Administration (FDA)
  4. Occupational Safety and Health Administration (OSHA)
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6.69) What federal agency approves disinfectants for use in laboratories?
  1. American Medical Association (AMA)
  2. Drug Enforcement Administration (DEA)
  3. Environmental Protection Agency (EPA)
  4. Food and Drug Administration (FDA)
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6.70) Which federal agency requires employers to provide workers with PPE?
  1. Centers for Disease Control and Prevention (CDC)
  2. National Institute for Occupational Safety and Health (NIOSH)
  3. National Institutes of Health (NIH)
  4. Occupational Safety and Health Administration (OSHA)
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6.71) Which federal agency is responsible for ensuring that clinical laboratories uphold and enforce CLIA standards?
  1. Center for Global Health (CGH)
  2. Centers for Medicare and Medicaid Services (CMS)
  3. Health Resources and Services Administration (HRSA)
  4. Office of Public Health Science (OPHS)
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6.72) Who enforces HIPAA?
  1. The Attorney General
  2. The Department of Health and Human Services
  3. The Surgeon General
  4. The US Senate
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6.73) Which federal agency enforces federal employment discrimination laws, such as the Civil Rights Act and the Americans with Disabilities Act (ADA)?
  1. Employee Benefits Security Administration (EBSA)
  2. Employment and Training Administration (ETA)
  3. Equal Employment Opportunity Commission (EEOC)
  4. Occupational Safety and Health Administration (OSHA)
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6.74) Which federal agency regulates the transport of hazardous materials?
  1. Centers for Disease Control and Prevention (CDC)
  2. Department Of Transportation (DOT)
  3. Food and Drug Administration (FDA)
  4. National Highway Traffic Safety Administration (NHTSA)
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6.75) Which federal agency regulates laboratory testing performed on humans?
  1. American Medical Association (AMA)
  2. Centers for Disease Control and Prevention (CDC)
  3. Centers for Medicare & Medicaid Service (CMS)
  4. Food and Drug Administration (FDA)
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6.76) What federal legislation regulates clinical laboratories?
  1. Clinical Laboratory Improvement Amendments (CLIA)
  2. Federal Clinical Laboratory Orders
  3. Good Clinical Laboratory Practice (GCLP) standards
  4. Laboratory Safety and Security Regulations
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6.77) Which act prevents employers from retaliating against workers for complaining about unsafe working conditions?
  1. Fair Labor Standards Act (FLSA)
  2. Federal Employees' Compensation Act (FCCA)
  3. Occupational Safety and Health Act (OSHA)
  4. Uniformed Services Employment and Reemployment Rights Act (USERRA)
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6.78) Which federal law requires health care providers to provide a sign language interpreter for deaf patients?
  1. Americans with Disabilities Act (ADA)
  2. Family and Medical Leave Act (FMLA)
  3. Health Care Right of Conscience Act (HCRCA)
  4. Health Insurance Portability and Accountability Act (HIPAA)
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6.79) Which act outlines the lawful use and disclosure of protected health information?
  1. Digital Accountability and Transparency Act (DATA)
  2. Freedom of Information Act (FOIA)
  3. Health Insurance Portability and Accountability Act (HIPAA)
  4. Privacy Act
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6.80) What worldwide system gives recommendations on how to label hazardous chemicals?
  1. GHS
  2. HCS
  3. HPR
  4. HazCom
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6.81) The Globally Harmonized System of Classification and Labeling of Chemicals has two signal words for hazardous chemicals: "Danger" and:
  1. Attention
  2. Caution
  3. Notice
  4. Warning
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6.82) How often does CLIA require laboratories to perform proficiency testing?
  1. Every month
  2. Every two years
  3. Once a year
  4. Twice a year
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6.83) Who or what does protective isolation protect?
  1. A patient
  2. Lab equipment
  3. Medical personnel
  4. The general public
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